Document Detail

An unusual desaturase in Aquilegia vulgaris.
MedLine Citation:
PMID:  11171154     Owner:  NLM     Status:  MEDLINE    
Aquilegia vulgaris seed oil contains high levels of the rare fatty acid columbinic acid (18:3 Delta(5,9,12)), which is unusual in having the double bond at the Delta(5) carbon in the trans configuration. Columbinic acid was found to be a seed-specific fatty acid not only present in the storage oil but also in membrane lipids. Several putative gene fragments have been isolated from plant RNA with sequences similar to previously characterized 'front-end' desaturases. Functional characterization of the Aquilegia cDNA is underway.
A J Longman; L V Michaelson; O Sayanova; J A Napier; A K Stobart
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  28     ISSN:  0300-5127     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-26     Completed Date:  2001-05-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  641-3     Citation Subset:  IM    
School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
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MeSH Terms
Binding Sites
Cytochromes b5 / metabolism
Fatty Acid Desaturases / genetics,  metabolism*
Linolenic Acids / analysis,  metabolism
Lipids / chemistry*
Membrane Lipids / chemistry
Open Reading Frames
Plant Oils / chemistry
Plants / enzymology*,  genetics
Seeds / chemistry
Reg. No./Substance:
0/Linolenic Acids; 0/Lipids; 0/Membrane Lipids; 0/Plant Oils; 13237-97-3/5,9,12-octadecatrienoic acid; 9035-39-6/Cytochromes b5; EC 1.14.19.-/Fatty Acid Desaturases

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