Document Detail


An unusual dependence of human herpesvirus-8 glycoproteins-induced cell-to-cell fusion on heparan sulfate.
MedLine Citation:
PMID:  19747451     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human herpesvirus-8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins-induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from following lines of evidence: (1) a significant reduction in cell-to-cell fusion occurred when target cells were treated with heparinase; (2) in a competition assay, when the effector cells expressing HHV-8 glycoproteins were challenged with soluble HS, cell-to-cell fusion was reduced; and, (3) co-expression of HHV-8 glycoproteins gH-gL on target cells resulted in inhibition of cell surface HS expression. Taken together, our results indicate that cell surface HS can play an additional role during HHV-8 pathogenesis.
Authors:
Vaibhav Tiwari; Nissar A Darmani; Gerald R Thrush; Deepak Shukla
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-09-09
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  390     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2009-12-29     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  382-7     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, University of Illinois at Chicago, IL 60612, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Fusion
Cell Membrane / metabolism,  virology
Cricetinae
Cricetulus
Heparitin Sulfate / metabolism*
Herpesviridae Infections / metabolism*
Herpesvirus 8, Human / metabolism*
Humans
Membrane Fusion*
Viral Envelope Proteins / genetics,  metabolism*
Viral Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
EY001792/EY/NEI NIH HHS; R01 AI057860/AI/NIAID NIH HHS; R01 AI057860-05/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Viral Envelope Proteins; 0/Viral Proteins; 0/glycoprotein B, human herpesvirus 8; 0/glycoprotein H, human herpesvirus 8; 0/glycoprotein L, human herpesvirus 8; 9050-30-0/Heparitin Sulfate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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