| An unsaturated fatty acid mutant of Aspergillus niger with partially defective delta 9-desaturase. | |
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MedLine Citation:
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PMID: 4005715 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The wild-type Aspergillus niger (V35) does not require fatty acids for growth. Four unsaturated fatty acid auxotrophs designated as UFA1, UFA2, UFA3, and UFA4 have been produced from this organism by treating the conidia of the wild-type strain with a mutagen, N-methyl-N'-nitro-N-nitrosoguanidine, followed by isolation on media containing monounsaturated fatty acids and the nonionic detergent, Brij 58. Optimal growth of the mutants comparable with that of the wild type was achieved with medium supplemented with C16 or C18 unsaturated fatty acids containing at least one cis double bond at the delta 9 position. Some other fatty acids (18:1 delta 11 cis and 16:1 delta 9 trans) support growth to some extent. The mutants do not grow at all in the presence of saturated fatty acids. Fatty acid analyses of the mutant, UFA2, grown in the presence of different fatty acid supplements reveal that it may be defective in a desaturase system. Experiments with unlabeled and [1-14C]palmitoyl-CoA have shown that the microsomes of the mutant (UFA2) contain a partially defective delta 9-desaturase system. |
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Authors:
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P Chattopadhyay; S K Banerjee; K Sen; P Chakrabarti |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 31 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 1985 Apr |
Date Detail:
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Created Date: 1985-08-12 Completed Date: 1985-08-12 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: CANADA |
Other Details:
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Languages: eng Pagination: 346-51 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus niger
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enzymology,
genetics*,
growth & development,
physiology Fatty Acid Desaturases / genetics*, metabolism Fatty Acids, Unsaturated / biosynthesis, pharmacology* Lipids / genetics Microsomes / enzymology Mutation |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Lipids; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-9 fatty acid desaturase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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