Document Detail


A universal analysis tool for the detection of asymmetric signal distribution in microscopic images.
MedLine Citation:
PMID:  22689329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Polarization of tissue is achieved by asymmetric distribution of proteins and organelles within individual cells. However, existing quantitative assays to measure this asymmetry in an automated and unbiased manner suffer from significant limitations.
RESULTS: Here, we report a new way to assess protein and organelle localization in tissue based on correlative fluorescence analysis. As a proof of principle, we successfully characterized planar cell polarity dependent asymmetry in developing Drosophila melanogaster tissues on the single cell level using fluorescence cross-correlation.
CONCLUSIONS: Systematic modulation of signal strength and distribution show that fluorescence cross-correlation reliably detects asymmetry over a broad parameter space. The novel method described here produces robust, rapid, and unbiased measurement of biometrical properties of cell components in live tissue that is readily applicable in other model systems.
Authors:
Maja Matis; Jeffrey D Axelrod; Milos Galic
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-23
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  241     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-18     Completed Date:  2012-11-28     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1301-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA. matis@stanford.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Polarity / physiology
Drosophila
Drosophila Proteins / metabolism*
Immunohistochemistry
Microscopy, Confocal
Microscopy, Fluorescence / methods*
Grant Support
ID/Acronym/Agency:
R01 GM059823/GM/NIGMS NIH HHS; R01 GM059823/GM/NIGMS NIH HHS; R01 GM098582/GM/NIGMS NIH HHS; R01 GM098582/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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