Document Detail


A universal HPLC method for the determination of phenolic acids in compound herbal medicines.
MedLine Citation:
PMID:  16104776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A universal method to separate and quantify 13 phenolic acids (gallic acid, chlorogenic acid, gentsic acid, vanillic acid, caffeic acid, syringic acid, sinapic acid, p-coumaric acid, ferulic acid, anisic acid, rosmarinic acid, salicylic acid, and cinnamic acid) in some compound herbal medicines was established by liquid chromatographic (HPLC). On an Agela XBP-C18 (5 microm, 4.6 mm x 150 mm) column, a multistep binary gradient elution program and a simplified sample pretreatment approach were used in the experiment. For all of the phenolic acids, detection limits ranged around 0.01 mg/L. Linear ranges of higher than 2 orders of magnitude were obtained with a correlation coefficient of 0.9991 to 1. Repeatability was 0.39-2.24% (relative standard deviation, RSD) for intraday, 1.17-3.96% (RSD) for interday, and 0.14-5.33% (RSD) for drug sample analysis. Recovery, tested by a standard addition method, ranged from 83.3% to 104.9% for various trace phenolic acids.
Authors:
Dawei Wen; Chenchen Li; Hao Di; Yiping Liao; Huwei Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-17     Completed Date:  2005-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6624-9     Citation Subset:  IM    
Affiliation:
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
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MeSH Terms
Descriptor/Qualifier:
Acids, Carbocyclic / analysis*
Caffeic Acids
Chromatography, High Pressure Liquid / methods*
Drugs, Chinese Herbal / analysis*
Hydroxybenzoic Acids / analysis
Quality Control
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Acids, Carbocyclic; 0/Caffeic Acids; 0/Drugs, Chinese Herbal; 0/Hydroxybenzoic Acids; 29656-58-4/phenolic acid; 331-39-5/caffeic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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