Document Detail


A unique tubulin antiserum attenuates the rate of poleward chromosome movement in anaphase.
MedLine Citation:
PMID:  1425771     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An antiserum against tubulin, NS20, was previously shown to specifically attenuate both fast axonal transport in vivo (Johnston, K. M. et al., Brain Res. 385, 38-45 (1986)) and in vitro (Johnston, K. M. et al., Cell Motil. Cytoskel. 7, 110-115 (1987)) and flagellar motility (Goldsmith, M. et al., Cell Motil. Cytoskel. 20, 249-262 (1991)). We hypothesized that NS20 blocked motility by binding to a multifunctional motor binding domain on the microtubules (MTs), or axonemes. Here we have examined the effect of microinjecting NS20, at metaphase, into dividing PtK2 cells. Plotting chromosome separation (CS) as a function of time, we report here that CS rates for anaphase A (chromosome-to-pole movement) were reduced by approximately 50% relative to uninjected controls. CS rates for anaphase B (spindle pole elongation) were unaffected by the NS20 antiserum. The inhibition of CS rate during anaphase A by NS20 was significantly greater than the inhibition caused by a control antitubulin serum (PC5). Two possible mechanisms underlying NS20's inhibition of CS during anaphase A were considered. NS20 could block the binding of a kinetochore-associated motor to kinetochore MTs (kMTs) or, alternatively, NS20 could stabilize kMTs against depolymerization. Our results favor the first alternative. In a cold-induced depolymerization assay, NS20 had no selective stabilizing effect on MTs. Moreover, we show that NS20 can selectively block the binding of a well characterized MT-associated motor (kinesin) to MTs, in vitro. These results suggest that NS20 may be defining a unique tubulin binding domain common to the motors underlying vesicle transport, flagellar motility, and chromosome movements during anaphase A.
Authors:
M Goldsmith; S Leyland; J A Connolly; D van der Kooy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cell biology     Volume:  58     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-12-23     Completed Date:  1992-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  346-55     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Faculty of Medicine, University of Toronto, Ontario, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anaphase / drug effects*
Animals
Antibodies / metabolism*
Binding Sites, Antibody
Cell Line
Chromosomes / drug effects
Kinesin / antagonists & inhibitors
Macropodidae
Microinjections
Microtubules / metabolism*
Tubulin / immunology*,  metabolism
Chemical
Reg. No./Substance:
0/Antibodies; 0/Binding Sites, Antibody; 0/Tubulin; EC 3.6.1.-/Kinesin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Macromolecular structure of reassembled neurofilaments as revealed by the quick-freeze deep-etch mic...
Next Document:  Evidence for reversible, non-microtubule and non-microfilament-dependent nuclear translocation of hs...