| A unique tubulin antiserum attenuates the rate of poleward chromosome movement in anaphase. | |
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MedLine Citation:
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PMID: 1425771 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An antiserum against tubulin, NS20, was previously shown to specifically attenuate both fast axonal transport in vivo (Johnston, K. M. et al., Brain Res. 385, 38-45 (1986)) and in vitro (Johnston, K. M. et al., Cell Motil. Cytoskel. 7, 110-115 (1987)) and flagellar motility (Goldsmith, M. et al., Cell Motil. Cytoskel. 20, 249-262 (1991)). We hypothesized that NS20 blocked motility by binding to a multifunctional motor binding domain on the microtubules (MTs), or axonemes. Here we have examined the effect of microinjecting NS20, at metaphase, into dividing PtK2 cells. Plotting chromosome separation (CS) as a function of time, we report here that CS rates for anaphase A (chromosome-to-pole movement) were reduced by approximately 50% relative to uninjected controls. CS rates for anaphase B (spindle pole elongation) were unaffected by the NS20 antiserum. The inhibition of CS rate during anaphase A by NS20 was significantly greater than the inhibition caused by a control antitubulin serum (PC5). Two possible mechanisms underlying NS20's inhibition of CS during anaphase A were considered. NS20 could block the binding of a kinetochore-associated motor to kinetochore MTs (kMTs) or, alternatively, NS20 could stabilize kMTs against depolymerization. Our results favor the first alternative. In a cold-induced depolymerization assay, NS20 had no selective stabilizing effect on MTs. Moreover, we show that NS20 can selectively block the binding of a well characterized MT-associated motor (kinesin) to MTs, in vitro. These results suggest that NS20 may be defining a unique tubulin binding domain common to the motors underlying vesicle transport, flagellar motility, and chromosome movements during anaphase A. |
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Authors:
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M Goldsmith; S Leyland; J A Connolly; D van der Kooy |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of cell biology Volume: 58 ISSN: 0171-9335 ISO Abbreviation: Eur. J. Cell Biol. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-12-23 Completed Date: 1992-12-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7906240 Medline TA: Eur J Cell Biol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 346-55 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Faculty of Medicine, University of Toronto, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anaphase
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drug effects* Animals Antibodies / metabolism* Binding Sites, Antibody Cell Line Chromosomes / drug effects Kinesin / antagonists & inhibitors Macropodidae Microinjections Microtubules / metabolism* Tubulin / immunology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Binding Sites, Antibody; 0/Tubulin; EC 3.6.1.-/Kinesin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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