| A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications. | |
| | |
MedLine Citation:
|
PMID: 22375034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Reversibly switchable fluorescent proteins (RSFPs) have attracted widespread interest for emerging techniques including repeated tracking of protein behavior and superresolution microscopy. Among the limited number of RSFPs available, only Dronpa is widely employed for most cell biology applications due to its monomeric and other favorable photochemical properties. Here we developed a series of monomeric green RSFPs with beneficial optical characteristics such as high photon output per switch, high photostability, a broad range of switching rate, and pH-dependence, which make them potentially useful for various applications. One member of this series, mGeos-M, exhibits the highest photon budget and localization precision potential among all green RSFPs. We propose mGeos-M as a candidate to replace Dronpa for applications such as dynamic tracking, dual-color superresolution imaging, and optical lock-in detection. |
| | |
Authors:
|
Hao Chang; Mingshu Zhang; Wei Ji; Juanjuan Chen; Yongdeng Zhang; Bei Liu; Jingze Lu; Junlong Zhang; Pingyong Xu; Tao Xu |
Related Documents
:
|
22315104 - Combined time- and space-resolved raman spectrometer for the non-invasive depth profili... 23262884 - 73.7 tb/s (96 x 3 x 256-gb/s) mode-division-multiplexed dp-16qam transmission with inli... 16127914 - Injection-locking laser-diode-based oc-192 optical non-return-to-zero-to-return-to-zero... 16642084 - Energy and average power scalable optical parametric chirped-pulse amplification in ytt... 19548764 - Robustness of integrin signaling network. 19794744 - Laser cooling of trapped ca(+) and measurement of the 3(2)d(5/2) state lifetime. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-02-28 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 109 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-03-21 Completed Date: 2012-05-09 Revised Date: 2012-10-01 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 4455-60 Citation Subset: IM |
Affiliation:
|
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Green Fluorescent Proteins / chemistry HeLa Cells Humans Hydrogen-Ion Concentration Kinetics Microscopy / methods Microscopy, Confocal / methods Microscopy, Fluorescence / instrumentation, methods* Mutation Photochemistry / methods Photons Spectrophotometry / methods |
| Chemical | |
Reg. No./Substance:
|
147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues ...
Next Document: Electrical resistivity and thermal conductivity of liquid Fe alloys at high P and T, and heat flux i...