Document Detail


A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications.
MedLine Citation:
PMID:  22375034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reversibly switchable fluorescent proteins (RSFPs) have attracted widespread interest for emerging techniques including repeated tracking of protein behavior and superresolution microscopy. Among the limited number of RSFPs available, only Dronpa is widely employed for most cell biology applications due to its monomeric and other favorable photochemical properties. Here we developed a series of monomeric green RSFPs with beneficial optical characteristics such as high photon output per switch, high photostability, a broad range of switching rate, and pH-dependence, which make them potentially useful for various applications. One member of this series, mGeos-M, exhibits the highest photon budget and localization precision potential among all green RSFPs. We propose mGeos-M as a candidate to replace Dronpa for applications such as dynamic tracking, dual-color superresolution imaging, and optical lock-in detection.
Authors:
Hao Chang; Mingshu Zhang; Wei Ji; Juanjuan Chen; Yongdeng Zhang; Bei Liu; Jingze Lu; Junlong Zhang; Pingyong Xu; Tao Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-02-28
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-21     Completed Date:  2012-05-09     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4455-60     Citation Subset:  IM    
Affiliation:
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Green Fluorescent Proteins / chemistry
HeLa Cells
Humans
Hydrogen-Ion Concentration
Kinetics
Microscopy / methods
Microscopy, Confocal / methods
Microscopy, Fluorescence / instrumentation,  methods*
Mutation
Photochemistry / methods
Photons
Spectrophotometry / methods
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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