Document Detail


A unique expression pattern of Tbx10 in the hindbrain as revealed by Tbx10(LacZ) allele.
MedLine Citation:
PMID:  20162674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study the expression/function of Tbx10, a T-box gene, Tbx10(LacZ/+) mice were established by replacing the T-box coding region with a LacZ gene. X-gal staining showed that LacZ(+) cells were localized to two-cell populations in rhombomere 4 and rhombomere 6. No significant differences in the locations of LacZ(+) cells were found between Tbx10(LacZ/+) and Tbx10(LacZ/LacZ) mice, and the Tbx10(LacZ/LacZ) mice were viable and fertile. We found that the LacZ(+) cells are present in both embryonic and adult mice. Histological studies suggest that the rhombomere 4-derived LacZ(+) cells are a subpopulation of the ventral interneurons in the pons.
Authors:
Xiao Dong Xue; Wataru Kimura; Bo Wang; Keisuke Hikosaka; Tatsuo Itakura; Tadayoshi Uezato; Masashi Matsuda; Haruhiko Koseki; Naoyuki Miura
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Publication Detail:
Type:  Letter; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  48     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-302     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Embryo, Mammalian / cytology,  embryology,  metabolism
Female
Gene Expression Profiling*
Gene Expression Regulation, Developmental*
Immunohistochemistry
In Situ Hybridization
Interneurons / cytology,  metabolism
Lac Operon / genetics
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Mice, Transgenic
Mutation
Pons / cytology,  embryology,  metabolism
Rhombencephalon / cytology,  embryology,  metabolism*
T-Box Domain Proteins / genetics*,  metabolism
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/T-Box Domain Proteins; 0/Tbx10 protein, mouse; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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