Document Detail


A unique N-glycan on human transferrin in CSF: a possible biomarker for iNPH.
MedLine Citation:
PMID:  21459485     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Idiopathic normal pressure hydrocephalus (iNPH) is an elderly dementia caused by abnormal metabolism in the cerebrospinal fluid (CSF). The tap test is the current basis for confirming iNPH, but it shows very low sensitivity, indicating that many patients who might be cured by a shunt operation will be missed. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, we found two transferrin isoforms: one had a unique N-glycan (Tf-1) whereas the other had N-glycan similar to that of serum transferrin (Tf-2). Glycan analyses revealed that Tf-1 had branching (biantennary) asialo- and agalacto-complex type N-glycans (N-acetylglucosamine [GlcNAc]-terminated glycans), which carried bisecting β1,4-N-acetylglucosamine and core α1,6-fucose. To examine glycoform whether changes in iNPH, we introduced the Tf-2/Tf-1 ratio as a diagnostic index, which minimized blot-to-blot variations in measurement. The Tf-2/Tf-1 ratios of iNPH patients are significantly higher than those of controls (p = 0.0019) and Alzheimer's patients (p = 0.0010). This suggests that the Tf-2/Tf-1 ratio could distinguish iNPH from Alzheimer's disease, and possibly other dementias. In conclusion, glycoform analysis has diagnostic potential in neurological diseases.
Authors:
Satoshi Futakawa; Kiyomitsu Nara; Masakazu Miyajima; Atsushi Kuno; Hiromi Ito; Hiroyuki Kaji; Keiro Shirotani; Takashi Honda; Yuriko Tohyama; Kyoka Hoshi; Yusuke Hanzawa; Shinobu Kitazume; Rie Imamaki; Katsutoshi Furukawa; Kazuhiro Tasaki; Hiroyuki Arai; Tatsuhiko Yuasa; Masafumi Abe; Hajime Arai; Hisashi Narimatsu; Yasuhiro Hashimoto
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-1
Journal Detail:
Title:  Neurobiology of aging     Volume:  -     ISSN:  1558-1497     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100437     Medline TA:  Neurobiol Aging     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Affiliation:
Department of Biochemistry, Fukushima Medical University School of Medicine, Fukushima, Japan.
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