Document Detail

An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase.
MedLine Citation:
PMID:  18830277     Owner:  NLM     Status:  MEDLINE    
A fosmid clone 37F10 containing an archaeal 16S rRNA gene was screened out from a metagenomic library of Pearl River sediment, southern China. Sequence analysis of the 35 kb inserted fragment of 37F10 found that it contains a single 16S rRNA gene belonging to Miscellaneous Crenarchaeotal Group (MCG) and 36 open reading frames (ORFs). One ORF (orf11) encodes putative bacteriochlorophyll a synthase (bchG) gene. Bacteriochlorophyll a synthase gene has never been reported in a member of the domain Archaea, in accordance with the fact that no (bacterio)-chlorophyll has ever been detected in any cultivated archaea. The putative archaeal bchG (named as ar-bchG) was cloned and heterologously expressed in Escherichia coli. The protein was found to be capable of synthesizing bacteriochlorophyll a by esterification of bacteriochlorophyllide a with phytyl diphosphate or geranylgeranyl diphosphate. Furthermore, phylogenetic analysis clearly indicates that the ar-bchG diverges before the bacterial bchGs. Our results for the first time demonstrate that a key and functional enzyme for bacteriochlorophyll a biosynthesis does exist in Archaea.
Jun Meng; Fengping Wang; Feng Wang; Yanping Zheng; Xiaotong Peng; Huaiyang Zhou; Xiang Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-02
Journal Detail:
Title:  The ISME journal     Volume:  3     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-23     Completed Date:  2009-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  106-16     Citation Subset:  IM    
School of Life Sciences, Xiamen University, Xiamen, Fujian, PR China.
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MeSH Terms
Carbon-Oxygen Ligases / genetics,  isolation & purification*,  metabolism*
Chlorophyllides / metabolism
Cloning, Molecular
Cluster Analysis
Crenarchaeota / enzymology*,  genetics
DNA, Archaeal / chemistry,  genetics
Diphosphates / metabolism
Escherichia coli / genetics
Gene Expression
Molecular Sequence Data
Open Reading Frames
Polyisoprenyl Phosphates / metabolism
Rivers / microbiology*
Sequence Analysis, DNA
Sequence Homology
Reg. No./Substance:
0/Chlorophyllides; 0/DNA, Archaeal; 0/Diphosphates; 0/Polyisoprenyl Phosphates; 0/phytyl diphosphate; 14897-06-4/chlorophyllide a; 6699-20-3/geranylgeranyl pyrophosphate; EC 6.1.-/Carbon-Oxygen Ligases; EC 6.1.-/bacteriochlorophyll a synthase

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