Document Detail

The ultraviolet light inactivation of phi-X174 bacteriophage at different wave lengths and pH's.
MedLine Citation:
PMID:  13750383     Owner:  NLM     Status:  MEDLINE    
The bacterial virus, PhiX174, which contains a single strand of DNA has been inactivated by different wave lengths of monochromatic ultraviolet light at pH 7, 2, and 12. The action spectra for inactivation at these three pH's all showed minima at 2400 A rather than at 2300 A, which is the characteristic absorption minimum of DNA. The shapes of the action spectra have been analyzed in terms of the effects of absorbed light on the pyrimidines and purines rather than the effect on nucleoprotein. In this interpretation the pyrimidines are at least 2 to 3 times more sensitive than the purines. The quantum yield for inactivation of the virus at 2650 A and pH 7 is 0.006. The quantum efficiency for quanta absorbed in the pyrimidines is 0.0085 and for the purines 0.0035. It is pointed out that action spectra for single- and double-stranded polynucleotides should have minima at different wave lengths, and that this difference may be used to distinguish between these two configurations in vivo.
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biophysical journal     Volume:  1     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1960 Sep 
Date Detail:
Created Date:  1961-12-01     Completed Date:  1998-11-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  29-41     Citation Subset:  OM    
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MeSH Terms
Bacteriophages / radiation effects*
Ultraviolet Rays*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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