Document Detail

An ultrastructural and systemic analysis of glycosaminoglycans in thyroid-associated ophthalmopathy.
MedLine Citation:
PMID:  9683947     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine the ultrastructural localisation of glycosaminoglycans (GAGs) in the extraocular muscles (EOMs) of patients with thyroid-associated ophthalmopathy (TAO) and to see whether the quantity and type of GAGs present in blood and urine are markers of the disease. METHODS: Biopsies of affected EOMs were taken and studied by transmission electron microscopy (TEM). These were either fixed conventional for TEM, or in 0.5% tannic acid and others for immunogold staining. Serum hyaluronan (HA) was measured using a radioimmunoassay in patients with TAO as well as control subjects, and urinary GAG levels assessed by photometric quantitation of hexuronic acid after reaction with carbazole. The excretion pattern of the urinary GAGs was determined by discontinuous electrophoresis. RESULTS: TEM showed that there is a marked expansion of the endomysial space in TAO EOM biopsies as compared with non-TAO strabismus specimens. This is caused by an increased number of collagen fibres, interspersed with a granular amorphous material surrounding striated collagen fibres shown to be hyaluronan by immunogold staining. In contrast, serum hyaluronan concentrations were similar in TAO and control patients, although there was a statistically significant difference in the urinary GAG excretion between the two groups of patients examined. By discontinuous electrophoresis, chondroitin sulphate and heparan sulphate were present in both patients and controls. CONCLUSION: GAGs and in particularly HA are present at the EOM level in patients with recently inactive TAO. However, serum levels of HA and urinary GAGs are not sensitive indicators for their presence within the EOMs.
A Pappa; P Jackson; J Stone; P Munro; P Fells; C Pennock; S Lightman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Eye (London, England)     Volume:  12 ( Pt 2)     ISSN:  0950-222X     ISO Abbreviation:  Eye (Lond)     Publication Date:  1998  
Date Detail:
Created Date:  1998-08-11     Completed Date:  1998-08-11     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  8703986     Medline TA:  Eye (Lond)     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
Moorfields Eye Hospital NHS Trust, London, UK.
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MeSH Terms
Autoimmune Diseases / metabolism*,  pathology
Biological Markers / analysis
Glycosaminoglycans / analysis*,  urine
Graves Disease / metabolism*,  pathology
Hyaluronic Acid / blood
Microscopy, Electron
Middle Aged
Oculomotor Muscles / chemistry*,  ultrastructure
Reg. No./Substance:
0/Biological Markers; 0/Glycosaminoglycans; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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