| An ultrastructural and systemic analysis of glycosaminoglycans in thyroid-associated ophthalmopathy. | |
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MedLine Citation:
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PMID: 9683947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine the ultrastructural localisation of glycosaminoglycans (GAGs) in the extraocular muscles (EOMs) of patients with thyroid-associated ophthalmopathy (TAO) and to see whether the quantity and type of GAGs present in blood and urine are markers of the disease. METHODS: Biopsies of affected EOMs were taken and studied by transmission electron microscopy (TEM). These were either fixed conventional for TEM, or in 0.5% tannic acid and others for immunogold staining. Serum hyaluronan (HA) was measured using a radioimmunoassay in patients with TAO as well as control subjects, and urinary GAG levels assessed by photometric quantitation of hexuronic acid after reaction with carbazole. The excretion pattern of the urinary GAGs was determined by discontinuous electrophoresis. RESULTS: TEM showed that there is a marked expansion of the endomysial space in TAO EOM biopsies as compared with non-TAO strabismus specimens. This is caused by an increased number of collagen fibres, interspersed with a granular amorphous material surrounding striated collagen fibres shown to be hyaluronan by immunogold staining. In contrast, serum hyaluronan concentrations were similar in TAO and control patients, although there was a statistically significant difference in the urinary GAG excretion between the two groups of patients examined. By discontinuous electrophoresis, chondroitin sulphate and heparan sulphate were present in both patients and controls. CONCLUSION: GAGs and in particularly HA are present at the EOM level in patients with recently inactive TAO. However, serum levels of HA and urinary GAGs are not sensitive indicators for their presence within the EOMs. |
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Authors:
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A Pappa; P Jackson; J Stone; P Munro; P Fells; C Pennock; S Lightman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Eye (London, England) Volume: 12 ( Pt 2) ISSN: 0950-222X ISO Abbreviation: Eye (Lond) Publication Date: 1998 |
Date Detail:
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Created Date: 1998-08-11 Completed Date: 1998-08-11 Revised Date: 2009-11-03 |
Medline Journal Info:
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Nlm Unique ID: 8703986 Medline TA: Eye (Lond) Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 237-44 Citation Subset: IM |
Affiliation:
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Moorfields Eye Hospital NHS Trust, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Autoimmune Diseases / metabolism*, pathology Biological Markers / analysis Biopsy Female Glycosaminoglycans / analysis*, urine Graves Disease / metabolism*, pathology Humans Hyaluronic Acid / blood Immunohistochemistry Male Microscopy, Electron Middle Aged Oculomotor Muscles / chemistry*, ultrastructure Radioimmunoassay |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Glycosaminoglycans; 9004-61-9/Hyaluronic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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