Document Detail


An ultrastructural study of histochemical staining of complex carbohydrates in the mouse posterior vitreous body.
MedLine Citation:
PMID:  6409847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The vitreous body contains complex carbohydrates that can be demonstrated morphologically. Vitreous hyaluronic acid is very soluble but it can be precipitated by cetylpyridinium chloride (CPC) while being cross-linked by glutaraldehyde. Oligosaccharide chains of vitreous glycoproteins are fixed with glutaraldehyde alone. Mouse eyes were fixed with glutaraldehyde or glutaraldehyde and CPC and the complex carbohydrates of the posterior vitreous cortex were studied by electron microscopy. Cationic dyes were used in the fixative or for block-staining on most fixed tissue blocks to allow detailed observations of complex carbohydrates. Most blocks were postfixed with OsO4. The hyaluronic-acid domain on vitreous collagen fibrils sequentially contracted and expanded in size with various histochemical manipulations. Contraction of the domain of hyaluronic acid generally indicates an increased charge density. OsO4 contributes considerable charge density upon forming osmate esters, but tissue postfixed with OsO4 contained large globular forms of hyaluronic acid rather than the small globules observed in non-osmicated preparations. A model is proposed to explain the seemingly paradoxical findings by reference to suggested mechanisms of polysaccharide-ligand-OsO4 interactions.
Authors:
R H Rhodes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Histochemistry     Volume:  78     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1983  
Date Detail:
Created Date:  1983-09-23     Completed Date:  1983-09-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  125-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrates / analysis*
Cetylpyridinium
Fixatives
Glutaral
Hyaluronic Acid / analysis
Lead
Mice
Osmium
Vitreous Body / analysis*,  ultrastructure
Grant Support
ID/Acronym/Agency:
EY-03828/EY/NEI NIH HHS; EY-04705/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Fixatives; 111-30-8/Glutaral; 7439-92-1/Lead; 7440-04-2/Osmium; 7773-52-6/Cetylpyridinium; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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