Document Detail


An ultrasensitive universal detector based on neutralizer displacement.
MedLine Citation:
PMID:  22824896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diagnostic technologies that can provide the simultaneous detection of nucleic acids for gene expression, proteins for host response and small molecules for profiling the human metabolome will have a significant advantage in providing comprehensive patient monitoring. Molecular sensors that report changes in the electrostatics of a sensor's surface on analyte binding have shown unprecedented sensitivity in the detection of charged biomolecules, but do not lend themselves to the detection of small molecules, which do not carry significant charge. Here, we introduce the neutralizer displacement assay that allows charge-based sensing to be applied to any class of molecule irrespective of the analyte charge. The neutralizer displacement assay starts with an aptamer probe bound to a neutralizer. When analyte binding occurs the neutralizer is displaced, which results in a dramatic change in the surface charge for all types of analytes. We have tested the sensitivity, speed and specificity of this system in the detection of a panel of molecules: (deoxy)ribonucleic acid, ribonucleic acid, cocaine, adenosine triphosphate and thrombin.
Authors:
Jagotamoy Das; Kristin B Cederquist; Alexandre A Zaragoza; Paul E Lee; Edward H Sargent; Shana O Kelley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-03
Journal Detail:
Title:  Nature chemistry     Volume:  4     ISSN:  1755-4349     ISO Abbreviation:  Nat Chem     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-24     Completed Date:  2012-10-29     Revised Date:  2013-01-04    
Medline Journal Info:
Nlm Unique ID:  101499734     Medline TA:  Nat Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  642-8     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analysis*
Aptamers, Nucleotide / chemistry*
Biosensing Techniques / instrumentation,  methods*
Cocaine / analysis*
Humans
Microscopy, Electron, Scanning
RNA / analysis*
Thrombin / analysis*
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 50-36-2/Cocaine; 56-65-5/Adenosine Triphosphate; 63231-63-0/RNA; EC 3.4.21.5/Thrombin
Comments/Corrections
Comment In:
Nanomedicine (Lond). 2012 Oct;7(10):1481
Nat Methods. 2012 Sep;9(9):870   [PMID:  23097787 ]
Nat Chem. 2012 Aug;4(8):595-7   [PMID:  22824887 ]

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