Document Detail


An ultra-pure in vitro phase synchrony method employing centrifugal elutriation and viable flow cytometric cell sorting.
MedLine Citation:
PMID:  6539665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present a method of synchronizing cells in G1-, S-, and G2M-phases employing sequential centrifugal elutriation and viable flow cytometric cell sorting of Hoechst-33342 stained Chinese hamster ovary cells. G1- and S-phase cells can be separated to greater than 99% homogeneity and G2-M to 70% purity. Most of the 30% contamination in the G2-M fraction was due to S-phase cells, whose reproductive integrity could be eliminated through the use of high specific activity 3H-TdR. There were minimal toxic effects or perturbations to growth following the selection procedures. The most significant limitation of this technique appears to be the rate of cell sorting, which, with current equipment, is approximately 3,000 cells per second.
Authors:
G C Rice; P N Dean; J W Gray; W C Dewey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytometry     Volume:  5     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-07-31     Completed Date:  1984-07-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  289-98     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division*
Cell Line
Centrifugation / methods*
Cricetinae
Cricetulus
Female
Flow Cytometry / methods*
Ovary
Grant Support
ID/Acronym/Agency:
CA 14533/CA/NCI NIH HHS; CA 31813/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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