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The ultimate Wright-Giemsa stain: 60 years in the making.
MedLine Citation:
PMID:  21395491     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract Wright-Giemsa staining is a common procedure that is performed routinely in hematology laboratories. Consistency in intra-laboratory staining quality is essential for accurate morphological interpretation of blood smears. Although the Wright-Giemsa stain can be challenging to perform, the methods illustrated here have provided consistent, high quality stains in the Special Hematology Laboratory at the University of Minnesota for over half a century. We outline methods for collecting blood specimens, preparing the slides and performing a Wright-Giemsa stain using our combination of reagents. Various techniques that have been passed down in our laboratory for troubleshooting suboptimally stained specimens are shared as well.
Authors:
K Dunning; Ao Safo
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  86     ISSN:  1473-7760     ISO Abbreviation:  Biotech Histochem     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  69-75     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota.
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