Document Detail


The type III system-secreted effector EspZ localizes to host mitochondria and interacts with the translocase of inner mitochondrial membrane 17b.
MedLine Citation:
PMID:  21947777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively) are attaching and effacing (A/E) bacterial pathogens that cause severe diarrheal disease worldwide. To cause disease, A/E pathogens require a type III secretion system, which facilitates transport of bacterial effector proteins directly into infected host cells. One of these effector proteins translocated by the type III secretion system, EspZ, is essential for A/E pathogen infection and functions to prevent rapid death of EPEC-infected cells. We further investigated the mechanism of EspZ-mediated protection of infected host cells and found that a severe decrease in host mitochondrial membrane potential (Δψ(m)) occurs concurrently with host cell lysis during infection with EPEC lacking EspZ (ΔespZ). It was also demonstrated that EspZ localizes to host cell mitochondria and interacts with the translocase of inner mitochondrial membrane 17b (TIM17b). In addition, host cell cytotoxicity was exacerbated in the absence of TIM17b during wild-type (WT) EPEC infection. The findings of this study together provide the first evidence that EspZ localizes to host mitochondria and that TIM17b contributes to protection against rapid cell death during EPEC infection.
Authors:
Stephanie R Shames; Matthew A Croxen; Wanyin Deng; B Brett Finlay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-26
Journal Detail:
Title:  Infection and immunity     Volume:  79     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-14     Completed Date:  2012-01-13     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4784-90     Citation Subset:  IM    
Affiliation:
Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Escherichia coli / classification,  metabolism*
Escherichia coli Proteins / genetics,  metabolism*
Gene Expression Regulation, Bacterial / physiology*
Humans
Mitochondria / metabolism*
Mitochondrial Membrane Transport Proteins / genetics,  metabolism*
RNA Interference
RNA, Small Interfering
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Escherichia coli Proteins; 0/EspZ protein, E coli; 0/Mitochondrial Membrane Transport Proteins; 0/RNA, Small Interfering; 0/translocase of inner mitochondrial membrane 17b protein, human
Comments/Corrections

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