Document Detail


The type III inositol 1,4,5-trisphosphate receptor is phosphorylated by cAMP-dependent protein kinase at three sites.
MedLine Citation:
PMID:  16107208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IP3 (inositol 1,4,5-trisphosphate) receptors form tetrameric, IP3-gated Ca2+ channels in endoplasmic reticulum membranes, and are substrates for several kinases, including PKA (cAMP-dependent protein kinase). Activation of PKA has been reported to either enhance or inhibit type III IP3 receptor Ca2+-channel activity, but, as yet, the sites of phosphorylation remain unknown. Here, we reveal that PKA phosphorylates the type III IP3 receptor at Ser916, Ser934 and Ser1832, and that, intriguingly, each site is located close to a putative surface-exposed peptide loop. Furthermore, we demonstrate that Ser934 is considerably more susceptible to PKA-dependent phoshorylation than either Ser916 or Ser1832. These findings define the sites at which the type III IP3 receptor is phosphorylated by PKA, and provide the basis for exploring the functional consequences of this regulatory event.
Authors:
Matthew D Soulsby; Richard J H Wojcikiewicz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  392     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-02     Completed Date:  2006-03-13     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  493-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Binding Sites
Calcium Channels / metabolism*
Calcium Signaling
Cell Line
Cyclic AMP-Dependent Protein Kinases / metabolism*
Humans
Inositol 1,4,5-Trisphosphate Receptors
Mice
Molecular Sequence Data
Phosphorylation
Rats
Receptors, Cytoplasmic and Nuclear / metabolism*
Sequence Homology, Amino Acid
Grant Support
ID/Acronym/Agency:
DK49194/DK/NIDDK NIH HHS; R01 DK049194/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/ITPR1 protein, human; 0/Inositol 1,4,5-Trisphosphate Receptors; 0/Receptors, Cytoplasmic and Nuclear; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases
Comments/Corrections

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