| A two-dimensional electrophoresis reference map for the bovine placenta during late pregnancy. | |
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MedLine Citation:
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PMID: 19943264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An understanding of bovine placental gene expression is essential for the study of animal reproductive physiology. Recent reports have found that placental abnormalities occur frequently in cloned bovines and mice. However, the molecular mechanisms underlying bovine placenta function remain unclear. Here, we present a preliminary description of the bovine placenta proteome. Proteins within the isoelectric point ranging from 4.0 to 7.0 and 6.0 to 9.0 were analyzed separately using 2-DE, using three replicates of bovine placenta. Approximately 2000 spots were detected in a placental 2-D gel stained with Coomassie blue. Subsequent excision of 380 spots from gels and MALDI-TOF MS analysis allowed the identification of 273 proteins. Our results revealed the composite profiles of key proteins in the bovine placenta during late pregnancy. These protein profiles will shed light on placental function during pregnancy and assist with functional analysis of the proteins. |
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Authors:
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Hong Rye Kim; Rong Xun Han; Jong Taek Yoon; Chang Sik Park; Dong Il Jin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteomics Volume: 10 ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-15 Completed Date: 2010-04-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 564-73 Citation Subset: IM |
Affiliation:
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Research Center for Transgenic Cloned Pigs, Chungnam National University, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Electrophoresis, Gel, Two-Dimensional / methods* Female Isoelectric Point Peptide Mapping* Placenta / chemistry* Pregnancy Proteome / analysis* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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