Document Detail


A two-dimensional electrophoresis reference map for the bovine placenta during late pregnancy.
MedLine Citation:
PMID:  19943264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An understanding of bovine placental gene expression is essential for the study of animal reproductive physiology. Recent reports have found that placental abnormalities occur frequently in cloned bovines and mice. However, the molecular mechanisms underlying bovine placenta function remain unclear. Here, we present a preliminary description of the bovine placenta proteome. Proteins within the isoelectric point ranging from 4.0 to 7.0 and 6.0 to 9.0 were analyzed separately using 2-DE, using three replicates of bovine placenta. Approximately 2000 spots were detected in a placental 2-D gel stained with Coomassie blue. Subsequent excision of 380 spots from gels and MALDI-TOF MS analysis allowed the identification of 273 proteins. Our results revealed the composite profiles of key proteins in the bovine placenta during late pregnancy. These protein profiles will shed light on placental function during pregnancy and assist with functional analysis of the proteins.
Authors:
Hong Rye Kim; Rong Xun Han; Jong Taek Yoon; Chang Sik Park; Dong Il Jin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  10     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  564-73     Citation Subset:  IM    
Affiliation:
Research Center for Transgenic Cloned Pigs, Chungnam National University, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Electrophoresis, Gel, Two-Dimensional / methods*
Female
Isoelectric Point
Peptide Mapping*
Placenta / chemistry*
Pregnancy
Proteome / analysis*
Time Factors
Chemical
Reg. No./Substance:
0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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