Document Detail

RaxH/RaxR: a two-component regulatory system in Xanthomonas oryzae pv. oryzae required for AvrXa21 activity.
MedLine Citation:
PMID:  15195943     Owner:  NLM     Status:  MEDLINE    
Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight, one of the most serious diseases in rice. X. oryzae pv. oryzae Philippine race 6 (PR6) strains are unable to establish infection in rice lines expressing the resistance gene Xa21. Although the pathogen-associated molecule that triggers the Xa21-mediated defense response (AvrXa21) is unknown, six rax (required for AvrXa21 activity) genes encoding proteins involved in sulfur metabolism and Type I secretion were recently identified. Here, we report on the identification of two additional rax genes, raxR and raxH, which encode a response regulator and a histidine protein kinase of two-component regulatory systems, respectively. Null mutants of PR6 strain PXO99 that are impaired in either raxR, raxH, or both cause lesions significantly longer and grow to significantly higher levels than does the wild-type strain in Xa21-rice leaves. Both raxR and raxH mutants are complemented to wild-type levels of AvrXa21 activity by introduction of expression vectors carrying raxR and raxH, respectively. These null mutants do not affect AvrXa7 and AvrXa10 activities, as observed in inoculation experiments with Xa7- and Xa10-rice lines. Western blot and raxR/gfp promoter-reporter analyses confirmed RaxR expression in X. oryzae pv. oryzae. The results of promoter-reporter studies also suggest that the previously identified raxSTAB operon is a target for RaxH/RaxR regulation. Characterization of the RaxH/RaxR system provides new opportunities for understanding the specificity of the X. oryzae pv. oryzae-Xa21 interaction and may contribute to the identification of AvrXa21.
Saul Burdman; Yuwei Shen; Sang-Won Lee; Qinzhong Xue; Pamela Ronald
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  17     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-15     Completed Date:  2004-09-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  602-12     Citation Subset:  IM    
Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel.
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MeSH Terms
Bacterial Proteins / genetics,  metabolism
DNA, Bacterial / chemistry
Escherichia coli / genetics
Gene Expression Regulation
Genes, Bacterial
Genetic Complementation Test
Molecular Sequence Data
Open Reading Frames
Oryza sativa / microbiology*
Plant Proteins / metabolism*
Promoter Regions, Genetic
Protein Kinases / genetics*,  metabolism
Protein-Serine-Threonine Kinases / metabolism*
Recombinant Proteins / isolation & purification,  metabolism
Trans-Activators / genetics*,  metabolism
Xanthomonas / genetics*,  metabolism,  pathogenicity
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Plant Proteins; 0/Recombinant Proteins; 0/Trans-Activators; 0/Xa21 protein, Oryza sativa; 0/avrXa10 protein, Xanthomonas; 0/avrXa7 protein, Xanthomonas oryzae; 0/osmolarity response regulator proteins; EC 2.7.-/Protein Kinases; EC Kinases; EC 2.7.3.-/protein-histidine kinase

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