| A two-compartment bioreactor system made of commercial parts for bioprocess scale down studies: Impact of oscillations on Bacillus subtilis fed-batch cultivations. | |
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MedLine Citation:
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PMID: 21751400 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study describes an advanced version of a two compartment scale-down bioreactor which simulates inhomogeneities present in large scale industrial bioreactors in the laboratory scale. The system is made of commercially available parts and is suitable for sterilization with steam. The scale-down bioreactor is comprised of a usual stirred tank bioreactor (STR) and a plug flow reactor (PFR) equipped with static mixer modules. The PFR module with a working volume of 1.2 L is equipped with five sample ports, pH and DO sensors. The concept is applied at the non-sporulating Bacillus subtilis mutant strain AS3 characterized by a SpoIIGA gene knockout. In a fed-batch process with a constant feed rate, it is found that oscillating substrate and dissolved oxygen concentration led to diminished glucose uptake, ethanol formation and an altered amino acid synthesis. Sampling at the plug flow reactor module allowed the detection of dynamics at concentrations of intermediates such as pyruvic acid and lactic acid as well as at amino acids. Results indicate that the carbon flux at excess glucose and low dissolved oxygen concentrations is shifted towards ethanol formation. As a result, the reduced carbon flux entering the TCA cycle is not sufficient to support amino acid synthesis following the oxaloacetic acid branch point. |
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Authors:
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Stefan Junne; Arne Klingner; Johannes Kabisch; Thomas Schweder; Peter Neubauer |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-13 |
Journal Detail:
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Title: Biotechnology journal Volume: - ISSN: 1860-7314 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101265833 Medline TA: Biotechnol J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Berlin, Germany. stefan.junne@tu-berlin.de. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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