| A two-colour fluorescence assay for the measurement of syncytial fusion between trophoblast-derived cell lines. | |
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MedLine Citation:
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PMID: 14580378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Syncytial fusion is a key event in implantation and placentation. Its regulation is only poorly understood. We present a cell-cell fusion assay based on staining of cells in two portions with a green and a red fluorescent cytoplasmic dye that become intracellularly mixed only after syncytial fusion. We quantified cell-cell fusion by fluorescence microscopy in choriocarcinoma cell lines BeWo, JAR and JEG3 and in some non-trophoblastic cell lines and found clear differences in fusion behaviour. Only BeWo cells fused with each other, while the other cell lines tested did not. BeWo cells also fused with all other cell lines tested. The efficiency of cell-cell fusion of BeWo cells was stimulated by forskolin. We tried to correlate messenger levels of syncytin and its receptor RDR with the fusion index of choriocarcinoma cells. BeWo and JAR cells contained readily detectable and forskolin-inducible levels of syncytin mRNA, whereas this messenger was barely detectable in JEG3 cells. RDR transcript levels were similar in all cell lines tested and were unaffected by forskolin treatment. The data suggests that the expression of syncytin and RDR messengers alone does not guarantee successful fusion. The fusion assay presented in this paper is a useful tool to study syncytial fusion in an accurate and quantitative way. |
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Authors:
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M Borges; P Bose; H-G Frank; P Kaufmann; A J G Pötgens |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Placenta Volume: 24 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-10-28 Completed Date: 2004-07-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 959-64 Citation Subset: IM |
Affiliation:
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Department of Anatomy II, University Hospital Aachen, Wendlingweg 2, D-52057 Aachen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Fusion Cell Line Cell Line, Tumor Color Fluorescent Dyes Giant Cells / cytology* Humans Microscopy, Fluorescence / methods* RNA / metabolism Trophoblasts / cytology*, metabolism, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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