Document Detail


A two-colour fluorescence assay for the measurement of syncytial fusion between trophoblast-derived cell lines.
MedLine Citation:
PMID:  14580378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Syncytial fusion is a key event in implantation and placentation. Its regulation is only poorly understood. We present a cell-cell fusion assay based on staining of cells in two portions with a green and a red fluorescent cytoplasmic dye that become intracellularly mixed only after syncytial fusion. We quantified cell-cell fusion by fluorescence microscopy in choriocarcinoma cell lines BeWo, JAR and JEG3 and in some non-trophoblastic cell lines and found clear differences in fusion behaviour. Only BeWo cells fused with each other, while the other cell lines tested did not. BeWo cells also fused with all other cell lines tested. The efficiency of cell-cell fusion of BeWo cells was stimulated by forskolin. We tried to correlate messenger levels of syncytin and its receptor RDR with the fusion index of choriocarcinoma cells. BeWo and JAR cells contained readily detectable and forskolin-inducible levels of syncytin mRNA, whereas this messenger was barely detectable in JEG3 cells. RDR transcript levels were similar in all cell lines tested and were unaffected by forskolin treatment. The data suggests that the expression of syncytin and RDR messengers alone does not guarantee successful fusion. The fusion assay presented in this paper is a useful tool to study syncytial fusion in an accurate and quantitative way.
Authors:
M Borges; P Bose; H-G Frank; P Kaufmann; A J G Pötgens
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Placenta     Volume:  24     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-28     Completed Date:  2004-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  959-64     Citation Subset:  IM    
Affiliation:
Department of Anatomy II, University Hospital Aachen, Wendlingweg 2, D-52057 Aachen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Cell Fusion
Cell Line
Cell Line, Tumor
Color
Fluorescent Dyes
Giant Cells / cytology*
Humans
Microscopy, Fluorescence / methods*
RNA / metabolism
Trophoblasts / cytology*,  metabolism,  ultrastructure
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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