Document Detail


The two actin-interacting protein 1 genes have overlapping and essential function for embryonic development in Caenorhabditis elegans.
MedLine Citation:
PMID:  21551072     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Disassembly of actin filaments by actin-depolymerizing factor (ADF)/cofilin and actin-interacting protein 1 (AIP1) is a conserved mechanism to promote reorganization of the actin cytoskeleton. We previously reported that unc-78, an AIP1 gene in the nematode Caenorhabditis elegans, is required for organized assembly of sarcomeric actin filaments in the body wall muscle. unc-78 functions in larval and adult muscle, and an unc-78-null mutant is homozygous viable and shows only weak phenotypes in embryos. Here we report that a second AIP1 gene, aipl-1 (AIP1-like gene-1), has overlapping function with unc-78, and that depletion of the two AIP1 isoforms causes embryonic lethality. A single aipl-1-null mutation did not cause a detectable phenotype. However, depletion of both unc-78 and aipl-1 arrested development at late embryonic stages due to severe disorganization of sarcomeric actin filaments in body wall muscle. In vitro, both AIPL-1 and UNC-78 preferentially cooperated with UNC-60B, a muscle-specific ADF/cofilin isoform, in actin filament disassembly but not with UNC-60A, a nonmuscle ADF/cofilin. AIPL-1 is expressed in embryonic muscle, and forced expression of AIPL-1 in adult muscle compensated for the function of UNC-78. Thus our results suggest that enhancement of actin filament disassembly by ADF/cofilin and AIP1 proteins is critical for embryogenesis.
Authors:
Shoichiro Ono; Kazumi Nomura; Sadae Hitosugi; Domena K Tu; Jocelyn A Lee; David L Baillie; Kanako Ono
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-05-05
Journal Detail:
Title:  Molecular biology of the cell     Volume:  22     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-04     Completed Date:  2011-12-12     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2258-69     Citation Subset:  IM    
Affiliation:
Department of Pathology, Emory University, Atlanta, GA 30322, USA. sono@emory.edu
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / metabolism
Animals
Caenorhabditis elegans / embryology*,  genetics*,  metabolism
Caenorhabditis elegans Proteins / genetics,  metabolism*
Destrin / metabolism
Embryonic Development / genetics*
Microfilament Proteins / genetics*,  metabolism
Muscles / metabolism
Mutation / genetics
Neurons / metabolism
Phenotype
Protein Isoforms / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
R01 AR48615/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Destrin; 0/Microfilament Proteins; 0/Protein Isoforms; 0/actin interacting protein 1
Comments/Corrections

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