| The tumour suppressor gene maspin is differentially regulated in cytotrophoblasts during human placental development. | |
| | |
MedLine Citation:
|
PMID: 11969337 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Identification of factors that play a role in regulating the highly invasive ability of human placental cells throughout gestation will contribute to a better understanding of this unique developmental process. The aims of this study were to determine whether the tumour suppressor gene maspin is present in the human placenta and plays a putative role in the regulation of cytotrophoblast invasion during placental development. The data showed that the expression of maspin mRNA was maximum in term placentae compared to the first and second trimester tissues, and absent in the HTR-SVneo (immortalized extravillous cytotrophoblast), JEG-3 and JAR (choriocarcinoma) cell lines. Maspin protein, detected by Western blot analysis, was twofold higher in the second trimester and 4.4-fold higher in the third trimester compared to the first trimester. Maspin immunohistochemical staining was localized in cytotrophoblasts with increased and more diffuse staining in the second and third trimesters. Corresponding to the period of maximum maspin expression, cytotrophoblasts isolated from term placentae had significantly lower invasive ability as compared to first and second trimester cytotrophoblasts (P< 0.03). Further, addition of recombinant maspin significantly decreased cytotrophoblast invasion in vitro by 40-50 per cent in all three trimesters of gestation. This study provides the first evidence of the temporal expression of maspin during human gestation and suggests a putative role for maspin in regulating the invasive activity of cytotrophoblasts at term. The down-regulation of maspin expression may be critical at the time of implantation and early placental development, whereas upregulation of maspin may serve as a signal for the end of cytotrophoblast invasion and gestation. |
| | |
Authors:
|
A Dokras; L M G Gardner; D A Kirschmann; E A Seftor; M J C Hendrix |
Related Documents
:
|
20455717 - A prospective, sequential and longitudinal study of haematological profile during norma... 14651517 - Human uterine leukocytes and pregnancy. 239427 - The existence during gestation of an immunological buffer zone at the interface between... 22523867 - Emergency contraception after sexual assault: changes in provision from 2004 to 2009. 20451187 - Pregnancy-related fibroid reduction. 20813877 - Leukotrienes modulate secretion of progesterone and prostaglandins during the estrous c... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Placenta Volume: 23 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2002 Apr |
Date Detail:
|
Created Date: 2002-04-23 Completed Date: 2002-11-12 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
|
Languages: eng Pagination: 274-80 Citation Subset: IM |
Copyright Information:
|
Copyright 2002 Elsevier Science Ltd. |
Affiliation:
|
Department of Obstetrics and Gynecology and Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA 52242, USA. anuja-dokras@uiowa.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Female Gene Expression Regulation, Developmental Genes, Tumor Suppressor* Humans Immunohistochemistry Placenta / growth & development*, metabolism* Pregnancy Pregnancy Trimester, Third Proteins / genetics*, metabolism*, pharmacology RNA, Messenger / genetics, metabolism Recombinant Proteins / pharmacology Serpins / genetics*, metabolism*, pharmacology Signal Transduction Trophoblasts / drug effects, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
5K12HD00849/HD/NICHD NIH HHS; CA 75681/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Proteins; 0/RNA, Messenger; 0/Recombinant Proteins; 0/SERPIN-B5; 0/Serpins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cytokines of the placenta and extra-placental membranes: roles and regulation during human pregnancy...
Next Document: Characterization of the expression of the retinoid-related, testis-associated receptor (RTR) in trop...