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A tumour cell aggregation promoting substance from rat ascites hepatoma cells.
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MedLine Citation:
PMID:  4374963     Owner:  NLM     Status:  MEDLINE    
A substance capable of promoting tumour cell aggregation was released from rat ascites hepatoma cell (possibly from the cell surface) kept in Hanks' balanced salt solution (free of calcium and magnesium) in the cold, and then partially purified by chromatography with DEAE-Sephadex and gel filtration with Bio-gel. The thermostable substance seemed to be a glycoprotein and its molecular weight was about 72,000 when measured by gel filtration on Sephadex G-200. It had no proteolytic activity. The material was clearly effective for rat ascites hepatoma cells as well as SV40 transformed cells, but less effective for Chang's cells and apparently ineffective for normal rat liver cells and red blood cells. The action of this material was more potent than that of Jack bean concanavalin A when assayed for aggregation of SV40 transformed cells. Its effect was not influenced by concanavalin A inhibitors such as alpha-methyl-D-glucopyranoside, N-acetyl-D-glucosamine and D-glucose.
K Kudo; I Tasaki; Y Hanaoka; H Hayashi
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  British journal of cancer     Volume:  30     ISSN:  0007-0920     ISO Abbreviation:  Br. J. Cancer     Publication Date:  1974 Dec 
Date Detail:
Created Date:  1975-05-13     Completed Date:  1975-05-13     Revised Date:  2011-07-20    
Medline Journal Info:
Nlm Unique ID:  0370635     Medline TA:  Br J Cancer     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  549-59     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Hepatocellular / analysis*
Cell Aggregation / drug effects*
Cell Separation
Cell Transformation, Neoplastic
Chromatography, Gel
Concanavalin A / pharmacology
Erythrocytes / drug effects
Glucosamine / pharmacology
Glucose / pharmacology
Glycoproteins / antagonists & inhibitors,  isolation & purification*,  pharmacology
Liver / drug effects
Liver Neoplasms / analysis
Methylglucosides / pharmacology
Molecular Weight
Simian virus 40
Tissue Extracts / pharmacology*
Reg. No./Substance:
0/Glycoproteins; 0/Methylglucosides; 0/Tissue Extracts; 11028-71-0/Concanavalin A; 3416-24-8/Glucosamine; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Br J Cancer
ISSN: 0007-0920
ISSN: 1532-1827
Article Information
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Print publication date: Month: 12 Year: 1974
Volume: 30 Issue: 6
First Page: 549 Last Page: 559
ID: 2009342
PubMed Id: 4374963
PubMed Id: 4374963

A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*
K. Kudo
I. Tasaki
Y. Hanaoka
H. Hayashi
*This is No. 1 of the studies on tumour cell aggregation promoting factor.

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