Document Detail

The tumor-derived fetal-intestinal alkaline phosphatase cDNA is identical in sequence to the adult intestinal alkaline phosphatase isozyme gene.
MedLine Citation:
PMID:  9352129     Owner:  NLM     Status:  MEDLINE    
The alkaline phosphatase (AP) of Caco-2 cells, a cell line derived from a human adenocarcinoma of the colon, is quite similar to fetal intestinal AP in its enzymatic properties. The nucleotide sequence of a cDNA encoding AP produced in Caco-2 cells was examined. The sequence was identical to one of the three sequences of adult intestinal AP reported previously. We further investigated the entire nucleotide sequence of cDNA of intestinal-type AP produced in cancer cell lines such as HuH-7 cells, FL-amnion cells, and HuG-1 cells. The sequence of these cell APs was identical to that of Caco-2 cell AP. These results indicate that cancer cells producing intestinal-type AP have the same nucleotide sequence as that of adult intestinal AP, and suggest that the differences in electrophoretic mobilities of these cell APs compared with adult intestinal AP may be due to post-translational modifications.
K Fukui; T Hada; H Imanishi; W Liu; A Iwasaki; K Hirano; K Higashino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  265     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-12-19     Completed Date:  1997-12-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  57-63     Citation Subset:  IM    
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
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MeSH Terms
Alkaline Phosphatase / genetics*
Amino Acid Sequence
Base Sequence
Caco-2 Cells / enzymology*
DNA, Complementary / analysis*,  genetics
Intestines / cytology,  enzymology*
Isoenzymes / genetics*
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA, Complementary; 0/Isoenzymes; EC Phosphatase

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