| The tumor-derived fetal-intestinal alkaline phosphatase cDNA is identical in sequence to the adult intestinal alkaline phosphatase isozyme gene. | |
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MedLine Citation:
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PMID: 9352129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The alkaline phosphatase (AP) of Caco-2 cells, a cell line derived from a human adenocarcinoma of the colon, is quite similar to fetal intestinal AP in its enzymatic properties. The nucleotide sequence of a cDNA encoding AP produced in Caco-2 cells was examined. The sequence was identical to one of the three sequences of adult intestinal AP reported previously. We further investigated the entire nucleotide sequence of cDNA of intestinal-type AP produced in cancer cell lines such as HuH-7 cells, FL-amnion cells, and HuG-1 cells. The sequence of these cell APs was identical to that of Caco-2 cell AP. These results indicate that cancer cells producing intestinal-type AP have the same nucleotide sequence as that of adult intestinal AP, and suggest that the differences in electrophoretic mobilities of these cell APs compared with adult intestinal AP may be due to post-translational modifications. |
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Authors:
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K Fukui; T Hada; H Imanishi; W Liu; A Iwasaki; K Hirano; K Higashino |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 265 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1997-12-19 Completed Date: 1997-12-19 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 57-63 Citation Subset: IM |
Affiliation:
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Third Department of Internal Medicine, Hyogo College of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Alkaline Phosphatase / genetics* Amino Acid Sequence Base Sequence Caco-2 Cells / enzymology* DNA, Complementary / analysis*, genetics Fetus Humans Intestines / cytology, enzymology* Isoenzymes / genetics* Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Isoenzymes; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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