Document Detail

A trypsin-like oviducal proteinase involved in Bufo arenarum fertilization.
MedLine Citation:
PMID:  34007     Owner:  NLM     Status:  MEDLINE    
When the jelly-less eggs removed from the most cephalic region of the oviduct (pars recta) of the toad Bufo arenarum were inseminated at a high sperm concentration, high frequencies of fertilization were obtained. On the other hand, control eggs removed from the pleuroperitoneal cavity (coelomic eggs)) were neither fertilized upon insemination under identical conditions, nor with the water extract of the jelly. Under these inseminating conditions, however, a high frequency of fertilization was obtained when coelomic eggs were preincubated in the presence of the fluid secreted by the epithelium of the pars recta or of an extract prepared from pars recta homogenate. Experimental evidence is presented showing that the component responsible for this effect acts on the vitelline envelope of the egg, increasing its susceptibility to sperm lysin. It is probable, therefore, that it induces successful fertilization of coelomic eggs by making the vitelline envelope more easily penetrable by sperm. The active factor was partially purified by Sephadex chromatography. The product obtained was of high activity and, as judged by its inhibition with soybean trypsin inhibitor and lima-bean trypsin inhibitor, it is likely to be a trypsin-like enzyme. The molecular weight of the factor was estimated to be 47000 by Sephadex chromatography. Secretion of the pars recta factor is hypophysis-dependent and its activity is not influenced by pH within the range testes (6.0--8.4).
D C Miceli; S N Fernández; J S Raisman; F D Barbieri
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of embryology and experimental morphology     Volume:  48     ISSN:  0022-0752     ISO Abbreviation:  J Embryol Exp Morphol     Publication Date:  1978 Dec 
Date Detail:
Created Date:  1979-05-23     Completed Date:  1979-05-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7906439     Medline TA:  J Embryol Exp Morphol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  79-91     Citation Subset:  IM    
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MeSH Terms
Bufo arenarum / physiology*
Hydrogen-Ion Concentration
Molecular Weight
Oviducts / enzymology*
Peptide Hydrolases / isolation & purification,  metabolism*
Spermatozoa / physiology
Reg. No./Substance:
EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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