Document Detail


A truncated HTLV-I envelope protein, lacking the hydrophobic membrane anchor domain, is associated with cellular membranes and virions.
MedLine Citation:
PMID:  8009867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The HTLV-I producer cell line C10/MJ2 does not induce syncytium formation with HTLV-I receptor expressing cells. Here we show that this cell line produces a truncated envelope protein, which, because of a premature stop codon, lacks the hydrophobic membrane anchor domain of the transmembrane protein (TM). Despite lacking a membrane anchor this envelope protein is expressed on the cell surface and associated with released virions. However, its incorporation into virions seems less efficient than that of a full-length envelope glycoprotein and some of its released into the cell culture supernatant as soluble surface glycoprotein (SU)-TM complexes. Small amounts of such a truncated envelope glycoprotein were also found in the fusion-competent HTLV-I producer cell line MT2. Premature truncation of HTLV-I envelope proteins in producer cell lines may result from in vitro selection for a less fusogenic phenotype. The association of truncated HTLV-I envelope proteins with virions and cell surfaces may reflect interactions between the SU domain and cellular membranes, possibly with the cellular receptor for HTLV-I.
Authors:
C V Carrington; R A Weiss; T F Schulz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  202     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-07-15     Completed Date:  1994-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-9     Citation Subset:  IM; X    
Affiliation:
Chester Beatty Laboratories, Institute of Cancer Research, London, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cell Line
Cell Membrane / microbiology*
Codon
DNA, Viral
Genes, env
Giant Cells / microbiology
Human T-lymphotropic virus 1 / immunology,  metabolism*
Humans
Molecular Sequence Data
Peptide Fragments / metabolism
T-Lymphocytes / microbiology
Viral Envelope Proteins / chemistry,  metabolism*
Virion*
Chemical
Reg. No./Substance:
0/Codon; 0/DNA, Viral; 0/Peptide Fragments; 0/Viral Envelope Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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