| The trolox analog, U-78517F, attenuates hypoxic injury in isolated cardiac myocytes. | |
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MedLine Citation:
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PMID: 7815467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neonatal rat cardiac myocytes were subjected to 5 h of hypoxia (PO2 < 5 mmHg) in glucose-free, modified Tyrodes solution. Prior to hypoxic exposure, cells were pretreated for 90 min with media alone or containing the trolox analog, U-78517F, at 0.3, 1, or 3 microM. Hypoxic injury was characterized by a significant loss of sarcolemmal integrity [76 +/- 12% of the total cell fluorescence (mean +/- S.E., n = 14 cultures)] quantified by nuclear staining with the membrane-impermeant fluorophore propidium iodide. In addition, hypoxia also induced significant decreases in the intracellular content of creatine kinase (43 +/- 7%, n = 12), lactate dehydrogenase (25 +/- 5%, n = 8), adenosine 5'-triphosphate (49 +/- 12%, n = 6), and sulfhydryls (34 +/- 4%, n = 6). Sarcolemmal integrity was significantly preserved by pretreatment with 1 microM and 3 microM U-78517F which reduced propidium iodide staining below the hypoxic control value by 51 +/- 9% (n = 12) and 75 +/- 4% (n = 14, P < 0.01 v hypoxic control), respectively. Similar results were obtained in cells pretreated with alpha-tocopherol suggesting that these two compounds are equipotent at preserving the sarcolemmal integrity of cardiac myocytes exposed to hypoxia.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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D A Linseman; T M Wall; J C Hartman |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of molecular and cellular cardiology Volume: 26 ISSN: 0022-2828 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 1994 Sep |
Date Detail:
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Created Date: 1995-02-03 Completed Date: 1995-02-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1249-57 Citation Subset: IM |
Affiliation:
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Upjohn Laboratories, Kalamazoo, MI 49001. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Antioxidants / pharmacology Cell Hypoxia / drug effects Chromans / pharmacology* Creatine Kinase / metabolism Heart / drug effects* L-Lactate Dehydrogenase / metabolism Myocardial Reperfusion Injury / metabolism, prevention & control Piperazines / pharmacology* Rats Rats, Sprague-Dawley Sarcolemma / drug effects, metabolism Sulfhydryl Compounds / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Chromans; 0/Piperazines; 0/Sulfhydryl Compounds; 133681-84-2/U 78517F; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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