Document Detail

The trolox analog, U-78517F, attenuates hypoxic injury in isolated cardiac myocytes.
MedLine Citation:
PMID:  7815467     Owner:  NLM     Status:  MEDLINE    
Neonatal rat cardiac myocytes were subjected to 5 h of hypoxia (PO2 < 5 mmHg) in glucose-free, modified Tyrodes solution. Prior to hypoxic exposure, cells were pretreated for 90 min with media alone or containing the trolox analog, U-78517F, at 0.3, 1, or 3 microM. Hypoxic injury was characterized by a significant loss of sarcolemmal integrity [76 +/- 12% of the total cell fluorescence (mean +/- S.E., n = 14 cultures)] quantified by nuclear staining with the membrane-impermeant fluorophore propidium iodide. In addition, hypoxia also induced significant decreases in the intracellular content of creatine kinase (43 +/- 7%, n = 12), lactate dehydrogenase (25 +/- 5%, n = 8), adenosine 5'-triphosphate (49 +/- 12%, n = 6), and sulfhydryls (34 +/- 4%, n = 6). Sarcolemmal integrity was significantly preserved by pretreatment with 1 microM and 3 microM U-78517F which reduced propidium iodide staining below the hypoxic control value by 51 +/- 9% (n = 12) and 75 +/- 4% (n = 14, P < 0.01 v hypoxic control), respectively. Similar results were obtained in cells pretreated with alpha-tocopherol suggesting that these two compounds are equipotent at preserving the sarcolemmal integrity of cardiac myocytes exposed to hypoxia.(ABSTRACT TRUNCATED AT 250 WORDS)
D A Linseman; T M Wall; J C Hartman
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  26     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1995-02-03     Completed Date:  1995-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1249-57     Citation Subset:  IM    
Upjohn Laboratories, Kalamazoo, MI 49001.
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MeSH Terms
Animals, Newborn
Antioxidants / pharmacology
Cell Hypoxia / drug effects
Chromans / pharmacology*
Creatine Kinase / metabolism
Heart / drug effects*
L-Lactate Dehydrogenase / metabolism
Myocardial Reperfusion Injury / metabolism,  prevention & control
Piperazines / pharmacology*
Rats, Sprague-Dawley
Sarcolemma / drug effects,  metabolism
Sulfhydryl Compounds / metabolism
Reg. No./Substance:
0/Antioxidants; 0/Chromans; 0/Piperazines; 0/Sulfhydryl Compounds; 133681-84-2/U 78517F; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; EC Dehydrogenase; EC Kinase

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