| A trial for induction of supernumerary primordial germ cells in Xenopus tadpoles by injecting RNA of Xenopus vasa homologue into germline cells of 32-cell embryos. | |
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MedLine Citation:
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PMID: 14706067 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Whether overexpression of Xenopus vasa homologue or Xenopus vasa-like gene 1 (XVLG1) in germline cells of Xenopus embryos can induce supernumerary primordial germ cells (PGC) at tadpole stage was investigated. XVLG1 RNA (0.1-2.0 ng) and beta-gal RNA (0.5 ng) were injected into one of, usually, four germ plasm-bearing cells (GPBC) of 32-cell embryos, with the beta-gal RNA (2.0 ng) serving as both lineage tracer and control for XVLG1 RNA. The total number of PGC, including X-gal-stained and unstained PGC of injected and uninjected GPBC origins respectively, was examined in the experimental tadpoles developed from the injected embryos. The injected RNA, XVLG1 and beta-gal RNA, were translated, resulting in a large amount of corresponding proteins in presumptive PGC (pPGC) as well as in somatic cells derived from the injected GPBC. Nevertheless, the average number of total PGC per tadpole found in the experimental tadpoles from the XVLG1 RNA-injected embryos was not significantly different from that of beta-gal RNA-injected ones, irrespective of the injected dose of XVLG1 RNA. This indicates that the extra XVLG1 protein in pPGC is not sufficient to increase the number of PGC in the tadpoles. |
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Authors:
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Kohji Ikenishi; Shinya Yamakita |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Development, growth & differentiation Volume: 45 ISSN: 0012-1592 ISO Abbreviation: Dev. Growth Differ. Publication Date: 2003 Oct-Dec |
Date Detail:
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Created Date: 2004-01-06 Completed Date: 2004-09-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0356504 Medline TA: Dev Growth Differ Country: Japan |
Other Details:
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Languages: eng Pagination: 417-26 Citation Subset: IM |
Affiliation:
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Department of Biology, Graduate School of Science, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi, Osaka 558-8585, Japan. ikenishi@sci.osaka-cu.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA, Complementary / metabolism Female Gene Library Germ Cells / cytology*, metabolism Male Models, Biological RNA / metabolism RNA, Messenger / metabolism Time Factors Xenopus / embryology, metabolism* Xenopus Proteins / metabolism* beta-Galactosidase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/RNA, Messenger; 0/XVLG1 protein, Xenopus; 0/Xenopus Proteins; 63231-63-0/RNA; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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