| A transposon encoding the complete 2,4-dichlorophenoxyacetic acid degradation pathway in the alkalitolerant strain Delftia acidovorans P4a. | |
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MedLine Citation:
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PMID: 12949179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bacterial strain Delftia acidovorans P4a, isolated from an extreme environment (heavily contaminated with organochlorines, highly alkaline conditions in an aqueous environment), was found to mineralize 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-methyl-4-chlorophenoxyacetic acid under alkaline conditions. Screening a genomic DNA library of the alkalitolerant strain for 2,4-D genes revealed the presence of the two 2,4-D gene clusters tfdCDEF and tfdC(II)E(II)BKA, tfdR genes being located in the vicinity of each tfd gene cluster. The results showed that the putative genes of the complete 2,4-D degradation pathway are organized in a single genomic unit. Sequence similarities to homologous gene clusters indicate that the individual tfd elements of strain P4a do not share a common origin, but were brought together by recombination events. The entire region is flanked by insertion elements of the IS1071 and IS1380 families, forming a transposon-like structure of about 30 kb, of which 28.4 kb were analysed. This element was shown to be located on the bacterial chromosome. The present study provides the first reported case of a chromosomally located catabolic transposon which carries the genes for the complete 2,4-D degradation pathway. |
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Authors:
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Doreen Hoffmann; Sabine Kleinsteuber; Roland H Müller; Wolfgang Babel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 149 ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-09-01 Completed Date: 2004-03-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
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Languages: eng Pagination: 2545-56 Citation Subset: IM |
Affiliation:
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UFZ Centre for Environmental Research, Department of Environmental Microbiology, Permoserstrasse 15, 04318 Leipzig, Germany. hoffmann@umb.ufz.de |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY078159 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dichlorophenoxyacetic Acid
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metabolism* Base Sequence Biodegradation, Environmental DNA Transposable Elements / genetics, physiology* Delftia acidovorans / classification, genetics, metabolism* Genes, Bacterial / physiology* Molecular Sequence Data Multigene Family |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; 94-75-7/2,4-Dichlorophenoxyacetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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