Document Detail

A transitional extracellular matrix instructs cell behavior during muscle regeneration.
MedLine Citation:
PMID:  20478295     Owner:  NLM     Status:  MEDLINE    
Urodele amphibians regenerate appendages through the recruitment of progenitor cells into a blastema that rebuilds the lost tissue. Blastemal formation is accompanied by extensive remodeling of the extracellular matrix. Although this remodeling process is important for appendage regeneration, it is not known whether the remodeled matrix directly influences the generation and behavior of blastemal progenitor cells. By integrating in vivo 3-dimensional spatiotemporal matrix maps with in vitro functional time-lapse imaging, we show that key components of this dynamic matrix, hyaluronic acid, tenascin-C and fibronectin, differentially direct cellular behaviors including DNA synthesis, migration, myotube fragmentation and myoblast fusion. These data indicate that both satellite cells and fragmenting myofibers contribute to the regeneration blastema and that the local extracellular environment provides instructive cues for the regenerative process. The fact that amphibian and mammalian myoblasts exhibit similar responses to various matrices suggests that the ability to sense and respond to regenerative signals is evolutionarily conserved.
Sarah Calve; Shannon J Odelberg; Hans-Georg Simon
Related Documents :
16889515 - A rapid seeding technique for the assembly of large cell/scaffold composite constructs.
16533765 - Kinetics of metastatic breast cancer cell trafficking in bone.
11353845 - Mechanical stress is communicated between different cell types to elicit matrix remodel...
11135375 - Identification and initial characterization of spore-like cells in adult mammals.
11172335 - Reconstitution of liver mass via cellular hypertrophy in the rat.
9067775 - Development of hassall's bodies of the thymus in humans and other vertebrates (especial...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-05-15
Journal Detail:
Title:  Developmental biology     Volume:  344     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-08-10     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-71     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Culture Techniques
Cell Movement
Extracellular Matrix / metabolism,  physiology*
Imaging, Three-Dimensional
Models, Biological
Muscle Development
Muscle Fibers, Skeletal / cytology
Muscle, Skeletal / cytology,  pathology
Muscles / pathology*
Oligonucleotide Array Sequence Analysis
Regeneration / physiology*
Stem Cells / cytology
Grant Support
T90 DA022881/DA/NIDA NIH HHS; T90 DA022881-04/DA/NIDA NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Re-programming of C. elegans male epidermal precursor fates by Wnt, Hox, and LIN-12/Notch activities...
Next Document:  Neural crest migration: patterns, phases and signals.