Document Detail


A transitional extracellular matrix instructs cell behavior during muscle regeneration.
MedLine Citation:
PMID:  20478295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Urodele amphibians regenerate appendages through the recruitment of progenitor cells into a blastema that rebuilds the lost tissue. Blastemal formation is accompanied by extensive remodeling of the extracellular matrix. Although this remodeling process is important for appendage regeneration, it is not known whether the remodeled matrix directly influences the generation and behavior of blastemal progenitor cells. By integrating in vivo 3-dimensional spatiotemporal matrix maps with in vitro functional time-lapse imaging, we show that key components of this dynamic matrix, hyaluronic acid, tenascin-C and fibronectin, differentially direct cellular behaviors including DNA synthesis, migration, myotube fragmentation and myoblast fusion. These data indicate that both satellite cells and fragmenting myofibers contribute to the regeneration blastema and that the local extracellular environment provides instructive cues for the regenerative process. The fact that amphibian and mammalian myoblasts exhibit similar responses to various matrices suggests that the ability to sense and respond to regenerative signals is evolutionarily conserved.
Authors:
Sarah Calve; Shannon J Odelberg; Hans-Georg Simon
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-05-15
Journal Detail:
Title:  Developmental biology     Volume:  344     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-08-10     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-71     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Movement
Extracellular Matrix / metabolism,  physiology*
Extremities
Imaging, Three-Dimensional
Models, Biological
Muscle Development
Muscle Fibers, Skeletal / cytology
Muscle, Skeletal / cytology,  pathology
Muscles / pathology*
Oligonucleotide Array Sequence Analysis
Regeneration / physiology*
Salamandridae
Stem Cells / cytology
Grant Support
ID/Acronym/Agency:
T90 DA022881/DA/NIDA NIH HHS; T90 DA022881-04/DA/NIDA NIH HHS
Comments/Corrections

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