Document Detail


A transformation booster sequence (TBS) from Petunia hybrida functions as an enhancer-blocking insulator in Arabidopsis thaliana.
MedLine Citation:
PMID:  19373469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several matrix-attachment regions (MARs) from animals have been shown to block interactions between an enhancer and promoter when situated between the two. Since a similar function for plant MARs has not been discerned, we tested the Zea mays ADH1 5' MAR, Nicotiana tabacum Rb7 3' MAR and a transformation booster sequence (TBS) MAR from Petunia hybrida for their ability to impede enhancer-promoter interactions in Arabidopsis thaliana. Stable transgenic lines containing vectors in which one of the three MAR elements or a 4 kb control sequence were interposed between the cauliflower mosaic virus 35S enhancer and a flower-specific AGAMOUS second intron-derived promoter (AGIP)::beta-glucuronidase (GUS) fusion were assayed for GUS expression in vegetative tissues. We demonstrate that the TBS MAR element, but not the ADH1 or Rb7 MARs, is able to block interactions between the 35S enhancer and AGIP without compromising the function of either with elements from which they are not insulated.
Authors:
Jean-Michel Hily; Stacy D Singer; Yazhou Yang; Zongrang Liu
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-04-17
Journal Detail:
Title:  Plant cell reports     Volume:  28     ISSN:  1432-203X     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-26     Completed Date:  2009-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1095-104     Citation Subset:  IM    
Affiliation:
USDA-ARS Appalachian Fruit Research Station, 2217 Wiltshire Road, Kearneysville, WV 25430, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics*,  metabolism
DNA, Plant / genetics
Enhancer Elements, Genetic*
Gene Expression Regulation, Plant
Genes, Plant
Genes, Reporter
Matrix Attachment Regions*
Petunia / genetics*
Plants, Genetically Modified / genetics,  metabolism
Promoter Regions, Genetic
Tobacco / genetics
Transformation, Bacterial
Zea mays / genetics
Chemical
Reg. No./Substance:
0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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