Document Detail


The transduction of His-TAT-p53 fusion protein into the human osteogenic sarcoma cell line (Saos-2) and its influence on cell cycle arrest and apoptosis.
MedLine Citation:
PMID:  17206471     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The p53 gene is a tumor suppressor gene. It encodes a nuclear phosphoprotein p53 involved in the regulation of cell cycle arrest and apoptosis to maintain the genomic integrity of the cell. As mutations of p53 gene are found in most human cancers, p53 protein becomes a hot target in the research of anticancer therapy. In the present study, an 11-amino acid domain of TAT protein which has been demonstrated to be able to transduce across cell membranes was fused with p53. The result revealed that the fusion protein His-TAT-p53 accumulated in the nucleus and inhibited the growth of the Saos-2 cells. Besides apoptosis, an increased percentage of G2 phase suggested that the transduction of His-TAT-p53 into cells might be associated with a G2 arrest of cell cycle.
Authors:
Lei Jiang; Yushu Ma; Jinzhi Wang; Xinyi Tao; Dongzhi Wei
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-06
Journal Detail:
Title:  Molecular biology reports     Volume:  35     ISSN:  0301-4851     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-01-22     Completed Date:  2008-03-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Cell Cycle*
Cell Line, Tumor
Cell Proliferation
Flow Cytometry
Fluorescent Antibody Technique
Gene Products, tat / metabolism*
Genetic Vectors
Humans
Osteosarcoma / metabolism,  pathology*
Plasmids
Protein Denaturation
Recombinant Fusion Proteins / isolation & purification,  metabolism*
Transduction, Genetic*
Tumor Suppressor Protein p53 / metabolism*
Chemical
Reg. No./Substance:
0/Gene Products, tat; 0/Recombinant Fusion Proteins; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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