Document Detail


The transcriptome of spermatozoa used in homologous intrauterine insemination varies considerably between samples that achieve pregnancy and those that do not.
MedLine Citation:
PMID:  19796764     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To differentiate transcripts' expression in the sperm from patients who achieved pregnancy in their first IUI cycle from those who did not. Basic sperm analysis is limited to forecasting pregnancies by means of assisted reproduction. New assays, such as microarray analysis, are potential predictive tools for this purpose. DESIGN: Nested case-control study. SETTING: University-affiliated private setting. PATIENT(S): Twenty sperm samples were obtained from infertile males undergoing their first IUI cycle with healthy partners. Sperm samples with which pregnancy was achieved (P; n=10) and those with which it was not achieved (NP; n=10) were identified and their respective messenger RNA expression profiles were compared. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Using microarrays, global genome expression was compared in pooled samples from each group. Results were evaluated to detect differentially expressed transcripts (TDEs; FC>2; P<0.05) and to identify those transcripts that were expressed in only one of the groups (exclusive transcripts [ETs]). RESULT(S): In group P, 756 TDEs presented increased expression, whereas 194 in group NP were found to be overexpressed. Furthermore, we found 741 ETs that were expressed only in group P and 976 that were expressed only in group NP. CONCLUSION(S): Results reveal profound differences between expression profiles of sperm samples that impregnate successfully and those that do not. These differences might improve the predictive power of sperm evaluation to estimate IUI success by complementing the basic sperm analysis.
Authors:
Sandra García-Herrero; Marcos Meseguer; José Antonio Martínez-Conejero; José Remohí; Antonio Pellicer; Nicolás Garrido
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-09-30
Journal Detail:
Title:  Fertility and sterility     Volume:  94     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-27     Completed Date:  2010-09-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1360-73     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Affiliation:
Instituto Universitario IVI Valencia, Universidad de Valencia, Valencia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adult
Case-Control Studies
Female
Fertilization / genetics*
Gene Expression Profiling*
Gene Expression Regulation
Humans
Infertility, Male / genetics*,  metabolism,  pathology
Insemination, Artificial, Homologous* / methods
Male
Oligonucleotide Array Sequence Analysis
Pregnancy
Pregnancy Rate
Semen Analysis
Spermatozoa / metabolism*,  pathology
Uterus

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