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The transcription termination site at the end of the early region of bacteriophage T7 DNA.
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MedLine Citation:
PMID:  7001354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nucleotide sequence through the transcription termination site for Escherichia coli RNA polymerase at the end of the early region of T7 DNA has been determined. RNA chains terminate at adjacent residues in the DNA sequence: about 2/3 of the chains end in C and 1/3 in G. A potential stem and loop structure, containing a stem of eight uninterrupted base pairs and a four-base loop, is centered 17-18 bases ahead of the chain termini. Transcription by E. coli RNA polymerase terminates efficiently at this site in vivo and in vitro, but transcription by T7 RNA polymerase is essentially unaffected. There are no primary cleavage sites for RNase III near the transcription termination site. The site of termination lies within HpaI fragment Q of T7 DNA, whose entire 446-nucleotide long sequence was determined. Cleavage sites for other restriction endonucleases are located conveniently for manipulating the DNA sequence around the termination site. The coding sequence for the last 82 amino acids of the T7 DNA ligase protein was identified, as was the beginning of a coding sequence for a possible late T7 protein from beyond the termination site.
Authors:
J J Dunn; F W Studier
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  8     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1980 May 
Date Detail:
Created Date:  1981-01-26     Completed Date:  1981-01-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2119-32     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J02518
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MeSH Terms
Descriptor/Qualifier:
Base Composition
Base Sequence
DNA, Viral / metabolism*
DNA-Directed RNA Polymerases / metabolism
Escherichia coli / metabolism
Nucleic Acid Conformation
Operon
Protein Biosynthesis
T-Phages / metabolism*
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/DNA, Viral; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 24 Month: 5 Year: 1980
Volume: 8 Issue: 10
First Page: 2119 Last Page: 2132
ID: 324067
PubMed Id: 7001354

The transcription termination site at the end of the early region of bacteriophage T7 DNA.
J J Dunn
F W Studier



Article Categories:
  • Research Article


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