Document Detail

The transcription factor six1 inhibits neuronal and promotes hair cell fate in the developing zebrafish (Danio rerio) inner ear.
MedLine Citation:
PMID:  17035528     Owner:  NLM     Status:  MEDLINE    
The developmental processes leading to the differentiation of mechanosensory hair cells and statoacoustic ganglion neurons from the early otic epithelium remain unclear. Possible candidates include members of the Pax-Six-Eya-Dach (paired box-sine oculis homeobox-eyes absent-dachshund) gene regulatory network. We cloned zebrafish six1 and studied its function in inner ear development. Gain- and loss-of-function experiments show that six1 has opposing roles in hair cell and neuronal lineages. It promotes hair cell fate and, conversely, inhibits neuronal fate by differentially affecting cell proliferation and cell death in these lineages. By independently targeting hair cells with atoh1a (atonal homolog 1a) knockdown or neurons with neurog1 (neurogenin 1) knockdown, we showed that the remaining cell population, neurons or hair cells, respectively, is still affected by gain or loss of six1 function. six1 interacts with other members of the Pax-Six-Eya-Dach regulatory network, in particular dacha and dachb in the hair cell but not neuronal lineage. Unlike in mouse, six1 does not appear to be dependent on eya1, although it seems to be important for the regulation of eya1 and pax2b expression in the ventral otic epithelium. Furthermore, six1 expression appears to be regulated by pax2b and also by foxi1 (forkhead box I1) as expected for an early inducer of the otic placode. Our results are the first to demonstrate a dual role for a member of the Pax-Six-Eya-Dach regulatory network in inner ear development.
Olivier Bricaud; Andres Collazo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  26     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-12     Completed Date:  2006-11-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10438-51     Citation Subset:  IM    
Leslie and Susan Gonda (Goldschmied) Cell and Molecular Biology Department, House Ear Institute, Los Angeles, California 90057, USA.
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MeSH Terms
Ear, Inner / embryology*,  metabolism
Gene Expression Regulation, Developmental / physiology
Hair Cells, Auditory, Inner / embryology*,  metabolism
Homeodomain Proteins / biosynthesis*,  genetics
Neural Inhibition / physiology*
Neurons / metabolism
Transcription Factors / biosynthesis*,  genetics
Zebrafish Proteins / biosynthesis*,  genetics
Reg. No./Substance:
0/Homeodomain Proteins; 0/Six1 protein, zebrafish; 0/Transcription Factors; 0/Zebrafish Proteins

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