Document Detail

The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II.
MedLine Citation:
PMID:  8090778     Owner:  NLM     Status:  MEDLINE    
The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. Substitution of either of these residues inactivates both TFIIS functions, suggesting a related role in both activities. These acidic residues may participate in phosphoryl transfer reactions by a two-metal-ion mechanism in a manner analogous to Klenow fragment. The RNA polymerase II itself may contain a Zn ribbon, in as much as the polymerase's 15-kDa subunit contains a sequence that aligns well with the TFIIS Zn ribbon sequence, including a similarly placed pair of acidic residues.
C Jeon; H Yoon; K Agarwal
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  91     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1994-10-14     Completed Date:  1994-10-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9106-10     Citation Subset:  IM    
Department of Biochemistry, University of Chicago, IL 60637.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Molecular Sequence Data
Mutagenesis, Site-Directed
RNA Polymerase II / metabolism*
RNA Processing, Post-Transcriptional
RNA, Messenger / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Structure-Activity Relationship
Transcription Factors / chemistry*,  metabolism
Transcription Factors, General*
Transcription, Genetic*
Transcriptional Elongation Factors*
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 0/Transcription Factors; 0/Transcription Factors, General; 0/Transcriptional Elongation Factors; 0/transcription factor S-II; 7440-66-6/Zinc; EC 2.7.7.-/RNA Polymerase II

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