Document Detail

The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells.
MedLine Citation:
PMID:  20505979     Owner:  NLM     Status:  MEDLINE    
Metastatic spreading of cancer cells is a highly complex process directed primarily by the interplay between tumor microenvironment, cell surface receptors, and actin cytoskeleton dynamics. To advance our understanding of metastatic cancer dissemination, we have developed a model system that is based on two v-src transformed chicken sarcoma cell lines-the highly metastatic parental PR9692 and a non-metastasizing but fully tumorigenic clonal derivative PR9692-E9. Oligonucleotide microarray analysis of both cell lines revealed that the gene encoding the transcription factor EGR1 was downregulated in the non-metastatic PR9692-E9 cells. Further investigation demonstrated that the introduction of exogenous EGR1 into PR9692-E9 cells restored their metastatic potential to a level indistinguishable from parental PR9692 cells. Microarray analysis of EGR1 reconstituted cells revealed the activation of genes that are crucial for actin cytoskeleton contractility (MYL9), filopodia formation (MYO10), the production of specific extracellular matrix components (HAS2, COL6A1-3) and other essential pro-metastatic abilities.
Vladimír Cermák; Jan Kosla; Jirí Plachý; Katerina Trejbalová; Jirí Hejnar; Michal Dvorák
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-28
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  67     ISSN:  1420-9071     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-21     Completed Date:  2010-10-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  3557-68     Citation Subset:  IM    
Institute of Molecular Genetics AS CR, v.v.i. Vídenská 1083, Prague, Czech Republic.
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MeSH Terms
Cell Adhesion
Cell Line
Cell Movement
Cell Proliferation
Cell Transformation, Neoplastic / genetics*,  pathology
Cytoskeleton / metabolism
Early Growth Response Protein 1 / genetics,  metabolism*
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Neoplasm Metastasis / genetics*
Oncogene Protein pp60(v-src) / genetics,  metabolism*
Sarcoma / genetics*,  pathology*
Reg. No./Substance:
0/Early Growth Response Protein 1; EC Protein pp60(v-src)

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