Document Detail


The trace analysis of alkyl alkylphosphonic acids in urine using gas chromatography-ion trap negative ion tandem mass spectrometry.
MedLine Citation:
PMID:  15664357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sensitive method has been developed for the trace analysis of alkyl alkylphosphonic acids, metabolites of nerve agents, in urine using a benchtop ion trap mass spectrometer. The acids were isolated from urine by simple solid phase extraction and converted to their pentafluorobenzyl esters. An ion trap mass spectrometer in selected reaction monitoring mode provided limits of detection of 0.1 ng/ml for isopropyl, isobutyl, pinacolyl and cyclohexyl methylphosphonic acids and for ethyl ethylphosphonic acid. The detection limit for ethyl methylphosphonic acid was higher (0.5 ng/ml) due to a lower recovery.
Authors:
James Riches; Ian Morton; Robert W Read; Robin M Black
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  816     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-24     Completed Date:  2005-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  251-8     Citation Subset:  IM    
Affiliation:
Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 OJQ, UK. jriches@dstl.gov.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / urine
Chemical Warfare Agents / analysis*
Gas Chromatography-Mass Spectrometry / methods*
Humans
Macaca mulatta
Phosphonic Acids / urine*
Sensitivity and Specificity
Soman / urine
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Chemical Warfare Agents; 0/Phosphonic Acids; 96-64-0/Soman

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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