| The toxic effects of pentachlorophenol on rat Sertoli cells in vitro. | |
| | |
MedLine Citation:
|
PMID: 21783587 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Pentachlorophenol (PCP) is the most toxic contaminant of chlorophenols (CPs). Due to improper disposal, PCP has become an environmental pollutant and is now considered to be ubiquitous. Previous studies about the influences of PCP on reproductive function were mostly focused on experiments in vivo. The aim of our present study was to estimate the toxic effects of PCP on cultured Sertoli cells from Sprague-Dawley rats. The viability of Sertoli cells was detected and morphological examination was performed followed by flow cytometric assay to evaluate its toxic effects. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that the toxic effects of PCP on cultured Sertoli cells were dose- and time-dependent. By flow cytometric assay, it was found that the number of dead Sertoli cells increased with the increase of exposed PCP levels. The results indicated that PCP had direct and dose-dependent cytotoxic effects on Sertoli cells in vitro. |
| | |
Authors:
|
Shuzhen Yang; Xiaodong Han; Chen Wei; Jianxiu Chen; Daqiang Yin |
Related Documents
:
|
8108117 - Ongoing mutations in the n-terminal domain of c-myc affect transactivation in burkitt's... 16709867 - Leukotriene a4 hydrolase expression in pel cells is regulated at the transcriptional le... 20736367 - Secreted and membrane-bound isoforms of protease adam9 have opposing effects on breast ... 22358967 - Single cell assay with an automated capillary microinjection system. 508287 - Endocytosis of lysosomal enzymes by non-parenchymal rat liver cells. comparative study ... 16449327 - Abp1 regulates pseudopodium number in chemotaxing dictyostelium cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Environmental toxicology and pharmacology Volume: 20 ISSN: 1382-6689 ISO Abbreviation: Environ. Toxicol. Pharmacol. Publication Date: 2005 Jul |
Date Detail:
|
Created Date: 2011-07-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9612020 Medline TA: Environ Toxicol Pharmacol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 182-7 Citation Subset: - |
Affiliation:
|
Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reduction in functional potency of the neurotoxin domoic acid in the presence of cadmium and zinc io...
Next Document: Disruption of normal embryonic angiogenesis by direct exposure of mainstream whole smoke solutions o...