Document Detail


toutvelu, a regulator of heparan sulfate proteoglycan biosynthesis, controls guidance cues for germ-cell migration.
MedLine Citation:
PMID:  17409068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The primitive embryonic gonad in Drosophila melanogaster is composed of germ cells and somatic gonadal precursor cells (SGPs). The assembly of a functional gonad involves a complex series of germ-cell migration events, which are thought to be guided by attractive and repulsive cues. Here, we demonstrate a novel role for toutvelu (ttv), a regulator of heparan sulfate proteoglycan biosynthesis during this process. Germline clonal analysis suggests that maternal deposition of ttv is required for proper germ-cell migration. Conversely, ectopic expression of ttv in early embryos results in severe germ-cell migration defects and inappropriate spreading of Hh protein. Moreover, overexpression of ttv in only the receiving cells, rather than in the sending cells, leads to phenotypic consequences. Finally, supporting the claim that the signaling molecule Hedgehog (Hh) may function as a chemoattractant to guide germ cells, errant germ cells are found localized near pockets containing high concentrations of Hh protein.
Authors:
Girish Deshpande; Nilay Sethi; Paul Schedl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-03
Journal Detail:
Title:  Genetics     Volume:  176     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-20     Completed Date:  2007-10-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  905-12     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08540, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement
Cues
Drosophila Proteins / genetics,  physiology*
Drosophila melanogaster / cytology,  embryology,  genetics,  physiology*
Female
Gene Expression Regulation
Germ Cells / cytology,  physiology*
Heparan Sulfate Proteoglycans / biosynthesis*
Immunohistochemistry
Membrane Proteins / genetics,  physiology*
Oviposition
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Heparan Sulfate Proteoglycans; 0/Membrane Proteins; 0/tout-velu protein, Drosophila
Comments/Corrections

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