| A total parenteral nutrition solution supplemented with a nucleoside and nucleotide mixture sustains intestinal integrity, but does not stimulate intestinal function after massive bowel resection in rats. | |
| | |
MedLine Citation:
|
PMID: 8598542 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effects of supplementing a total parenteral nutrition solution with a nucleoside and nucleotide mixture on mucosal adaptive processes after massive bowel resection were studied. Male Wistar rats (n=30) underwent 80% small intestine resection, were randomized into two groups and received either standard total parenteral nutrition (TPN) or TPN supplemented with a nucleoside and nucleotide mixture (2.5 mL.kg-1.d-1). An additional five rats, fed a nonpurified diet and not resected, were used as controls. After 4 or 7 d, rats were killed and samples were collected for mucosal indices and intestinal enzymatic activities (disaccharidases and diamine oxidase). After massive small bowel resection and TPN, residual jejunal mucosal wet weights, villus heights, protein and RNA contents on d 4 and 7, and total wet weights and DNA contents on d 7 were significantly lower than in the control group. Administration of the nucleoside and nucleotide mixture resulted in significantly higher residual jejunal total and mucosal weights, proteins, DNA, RNA contents, and the ratio of proliferating cell nuclear antigen positive cells per crypt than did the standard TPN solution on d 7. However, disaccharidase and diamine oxidase activities were not affected by supplementation with the nucleoside and nucleotide mixture. Our data suggest the supplementation of a nucleoside and nucleotide mixture to a TPN solution can attenuate the initial mucosal atrophy and improve intestinal cell turnover after massive bowel resection, but the supplementation has little effect on enterocyte enzymatic activities. |
| | |
Authors:
|
S Iijima; T Tsujinaka; M Kishibuchi; Y Kido; C Ebisui; K Kan; M Yano; T Mori |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of nutrition Volume: 126 ISSN: 0022-3166 ISO Abbreviation: J. Nutr. Publication Date: 1996 Mar |
Date Detail:
|
Created Date: 1996-04-25 Completed Date: 1996-04-25 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0404243 Medline TA: J Nutr Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 589-95 Citation Subset: IM |
Affiliation:
|
Department of Surgery II, Osaka University Medical School, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amine Oxidase (Copper-Containing)
/
analysis Animals Atrophy Body Weight DNA / analysis Disaccharidases / analysis Energy Intake Intestinal Mucosa / chemistry, drug effects, pathology Intestine, Small / surgery* Jejunum / chemistry, drug effects*, pathology Male Nucleosides / administration & dosage, pharmacology* Nucleotides / administration & dosage, pharmacology* Organ Size Parenteral Nutrition, Total* / adverse effects, standards Proteins / analysis RNA / analysis Random Allocation Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
0/Nucleosides; 0/Nucleotides; 0/Proteins; 63231-63-0/RNA; 9007-49-2/DNA; EC 1.4.3.6/Amine Oxidase (Copper-Containing); EC 3.2.1.-/Disaccharidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chemical modification of bacterial 4-aminobutyrate aminotransferase by phenylglyoxal.
Next Document: Development of insulin resistance in the rat is dependent on the rate of glucose absorption from the...