Document Detail


The torYZ (yecK bisZ) operon encodes a third respiratory trimethylamine N-oxide reductase in Escherichia coli.
MedLine Citation:
PMID:  11004177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bisZ gene of Escherichia coli was previously described as encoding a minor biotin sulfoxide (BSO) reductase in addition to the main cytoplasmic BSO reductase, BisC. In this study, bisZ has been renamed torZ based on the findings that (i) the torZ gene product, TorZ, is able to reduce trimethylamine N-oxide (TMAO) more efficiently than BSO; (ii) although TorZ is more homologous to BisC than to the TMAO reductase TorA (63 and 42% identity, respectively), it is located mainly in the periplasm as is TorA; (iii) torZ belongs to the torYZ operon, and the first gene, torY (formerly yecK), encodes a pentahemic c-type cytochrome homologous to the TorC cytochrome of the TorCAD respiratory system. Furthermore, the torYZ operon encodes a third TMAO respiratory system, with catalytic properties that are clearly different from those of the TorCAD and the DmsABC systems. The torYZ and the torCAD operons may have diverged from a common ancestor, but, surprisingly, no torD homologue is found in the sequences around torYZ. Moreover, the torYZ operon is expressed at very low levels under the conditions tested, and, in contrast to torCAD, it is not induced by TMAO or dimethyl sulfoxide.
Authors:
S Gon; J C Patte; V Méjean; C Iobbi-Nivol
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  182     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-26     Completed Date:  2000-10-26     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5779-86     Citation Subset:  IM    
Affiliation:
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, Centre Nationale de la Recherche Scientifique, 13402 Marseille Cedex 20, France.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Anaerobiosis
Chromosome Mapping
Escherichia coli / enzymology*,  genetics*,  growth & development
Isoenzymes / genetics,  isolation & purification,  metabolism
Kinetics
Molecular Sequence Data
NADH, NADPH Oxidoreductases / genetics*,  isolation & purification,  metabolism
Operon*
Oxidoreductases / genetics*,  isolation & purification,  metabolism
Oxidoreductases Acting on CH-NH Group Donors
Oxidoreductases, N-Demethylating / genetics*,  isolation & purification,  metabolism
Plasmids
Recombinant Proteins / isolation & purification,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Recombinant Proteins; EC 1.-/Oxidoreductases; EC 1.-/biotin sulfoxide reductase; EC 1.4.9.1/methylamine dehydrogenase; EC 1.5.-/Oxidoreductases Acting on CH-NH Group Donors; EC 1.5.-/Oxidoreductases, N-Demethylating; EC 1.5.8.2/trimethylamine dehydrogenase; EC 1.6.-/NADH, NADPH Oxidoreductases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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