Document Detail

A top-down analysis of Xa- and Xi-territories reveals differences of higher order structure at ≥ 20 Mb genomic length scales.
MedLine Citation:
PMID:  21970989     Owner:  NLM     Status:  MEDLINE    
The active and inactive X (Xa;Xi) territory with its seemingly highly compacted Barr body in nuclei of female mammalian cells provide a key example for studies of structure/function relationships in homologous chromosomes with different functional properties. Here we used about 300 human X-specific large insert clones to generate probe sets, which target physically or functionally defined sub-chromosomal segments. We combined 3D multicolor FISH with quantitative 3D image analysis in order to compare the higher order organization in Xi-and Xa-territories in human diploid fibroblasts (HDFs) at various length scales ranging from about 50 Mb down to 1 Mb. Xi-territories were characterized by a rounder shape as compared to the flatter and more extended shape of Xa-territories. The overall compaction of the entire Xi-territory, including the Barr body, was only 1.2-fold higher than the Xa-territory. Significant differences, however, were noted between distinct subchromosomal segments: At 20 Mb length scales higher compaction in Xi-territories was restricted to specific segments, but higher compaction in these segments was not correlated with gene density, transcriptional activity, LINE content or histone markers locally enriched in Xi-territories. Notably, higher compaction in Xi-territories observed for 20 Mb segments was not reflected accordingly by inclosed segments of 1-4 Mb. We conclude that compaction differences result mainly from a regrouping of ~1 Mb chromatin domains rather than from an increased condensation of individual domains. In contrast to a previous report, genes subject to inactivation as well as escaping from inactivation were not excluded from the interior of the Barr body.
Kathrin Teller; Doris Illner; Stefanie Thamm; Corella S Casas-Delucchi; Rogier Versteeg; Mireille Indemans; Thomas Cremer; Marion Cremer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-01
Journal Detail:
Title:  Nucleus (Austin, Tex.)     Volume:  2     ISSN:  1949-1042     ISO Abbreviation:  Nucleus     Publication Date:    2011 Sep-Oct
Date Detail:
Created Date:  2011-10-28     Completed Date:  2012-03-06     Revised Date:  2014-10-10    
Medline Journal Info:
Nlm Unique ID:  101518322     Medline TA:  Nucleus     Country:  United States    
Other Details:
Languages:  eng     Pagination:  465-77     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / ultrastructure
Cells, Cultured
Chromosome Mapping
Chromosomes, Human, X / ultrastructure*
DNA Probes / chemistry
Image Processing, Computer-Assisted
In Situ Hybridization, Fluorescence
RNA, Long Noncoding
RNA, Untranslated / analysis,  ultrastructure*
Sex Chromatin / ultrastructure
Reg. No./Substance:
0/DNA Probes; 0/RNA, Long Noncoding; 0/RNA, Untranslated; 0/XIST non-coding RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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