| A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response. | |
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MedLine Citation:
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PMID: 20358223 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Abiotic stresses such as cold, water deficit, and salt stresses severely reduce crop productivity. Tomato (Solanum lycopersicum) is an important economic crop; however, not much is known about its stress responses. To gain insight into stress-responsive gene regulation in tomato plants, we identified transcription factors from a tomato cDNA microarray. An ABA-responsive element binding protein (AREB) was identified and named SlAREB. In tomato protoplasts, SlAREB transiently transactivated luciferase reporter gene expression driven by AtRD29A (responsive to dehydration) and SlLAP (leucine aminopeptidase) promoters with exogenous ABA application, which was suppressed by the kinase inhibitor staurosporine, indicating that an ABA-dependent post-translational modification is required for the transactivation ability of SlAREB protein. Electrophoretic mobility shift assays showed that the recombinant DNA-binding domain of SlAREB protein is able to bind AtRD29A and SlLAP promoter regions. Constitutively expressed SlAREB increased tolerance to water deficit and high salinity stresses in both Arabidopsis and tomato plants, which maintained PSII and membrane integrities as well as water content in plant bodies. Overproduction of SlAREB in Arabidopsis thaliana and tomato plants regulated stress-related genes AtRD29A, AtCOR47, and SlCI7-like dehydrin under ABA and abiotic stress treatments. Taken together, these results show that SlAREB functions to regulate some stress-responsive genes and that its overproduction improves plant tolerance to water deficit and salt stress. |
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Authors:
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Tsai-Hung Hsieh; Chia-Wen Li; Ruey-Chih Su; Chiu-Ping Cheng; Sanjaya; Yi-Chien Tsai; Ming-Tsair Chan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-01 |
Journal Detail:
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Title: Planta Volume: 231 ISSN: 1432-2048 ISO Abbreviation: Planta Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 1459-73 Citation Subset: IM |
Affiliation:
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Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abscisic Acid
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pharmacology Adaptation, Physiological / drug effects, genetics Amino Acid Sequence Arabidopsis / drug effects, genetics Arabidopsis Proteins / metabolism Basic-Leucine Zipper Transcription Factors / chemistry, genetics, metabolism* DNA, Plant / metabolism Gene Expression Regulation, Plant / drug effects Genes, Reporter / genetics Luciferases / metabolism Lycopersicon esculentum / drug effects*, genetics, physiology* Molecular Sequence Data Plant Proteins / chemistry, genetics, metabolism Protein Binding / drug effects Protein Structure, Tertiary Protoplasts / metabolism Response Elements / genetics Salinity Signal Transduction / drug effects Sodium Chloride / pharmacology* Stress, Physiological / drug effects* Transcriptional Activation / drug effects, genetics Transgenes / genetics Water / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Basic-Leucine Zipper Transcription Factors; 0/DNA, Plant; 0/Plant Proteins; 0/RD29a protein, Arabidopsis; 134711-03-8/dehydrin proteins, plant; 21293-29-8/Abscisic Acid; 7647-14-5/Sodium Chloride; 7732-18-5/Water; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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