Document Detail


A tissue engineered heart valve implanted in a juvenile sheep model.
MedLine Citation:
PMID:  12709659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The tissue-engineered (TE) heart valve was developed to improve the durability of tissue heart valves. The aim of this study was to evaluate morphological and histological changes in a TE heart valve consisting of decellularized porcine matrices seeded with viable autologous vascular endothelial cells (AVEC). MATERIAL/METHODS: TE valves were implanted into the right ventricular outflow tract of eight juvenile sheep and explanted after 7 days, 3 and 6 months. The valves were evaluated visually, by invasive pressure measurements, X-ray, light microscopy, scanning, and transmission electron microscopy. The calcium content of the cusps was determined quantitatively by atomic absorption spectrometry. RESULTS: After valve implantation, all animals showed fast recovery with no complications during the observation period. Invasive pressure measurements presented a mean TE valve pressure gradient of 1.5+/-0.5 mm Hg at 3 and the same at 6 months. Light microscopy showed a monolayer of AVEC on all explanted heart valves, confirmed by scanning electron microscopy and immunohistochemical staining. X-ray examination of explanted TE heart valves showed no cusp calcification, confirmed by atomic absorption spectrometry. CONCLUSIONS: All explanted TE heart valves showed AVEC at the inner surface and ingrowth of fibroblast into the decellularized matrix, increasing during the observation period. The calcium contents were very low at explantation in these viable new heart valves.
Authors:
Pascal M Dohmen; Shigeyuki Ozaki; Robert Nitsch; Jessa Yperman; Willem Flameng; Wolfgang Konertz
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  9     ISSN:  1234-1010     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-23     Completed Date:  2003-08-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  BR97-BR104     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Surgery, Charit , Humboldt University Berlin, Berlin, Germany. pascal.dohmen@charite.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Bioprosthesis*
Calcium / analysis
Cells, Cultured
Endothelium, Vascular / cytology,  ultrastructure
Extracellular Matrix / metabolism
Follow-Up Studies
Heart Valve Prosthesis*
Heart Valve Prosthesis Implantation*
Immunohistochemistry
Models, Animal
Pulmonary Valve / surgery,  ultrastructure
Sheep
Spectrophotometry, Atomic
Time Factors
Tissue Engineering*
Chemical
Reg. No./Substance:
7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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