| The tip link's role in asymmetric stereocilia motion of chick cochlear hair cells. | |
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MedLine Citation:
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PMID: 9925012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The symmetry of chick cochlear hair bundle motion was examined in this study. Isolated segments from the basilar papilla were incubated in vitro in either normal or low calcium medium, which is known to disrupt tip links. Stereociliary bundles, stimulated with an oscillating water microjet, were oriented in profile and viewed in slow motion at high magnification with stroboscopic illumination. The displacement of the tallest hair in the bundle was fixed to 20 degrees peak-to-peak (P-P) motion. The angular deflections of the shortest and tallest hairs were then measured in both the positive (towards the tallest hair) and negative (towards the shortest) directions with respect to the non-stimulated position of the hair. The tallest hairs exhibited nearly symmetric motion in medium containing normal and low calcium. The shortest hairs, in normal calcium, displayed considerable asymmetry with angular deflections in the positive direction significantly larger than in the negative direction. This asymmetric motion disappeared after incubation in low calcium. The shortest hair angular displacement in the negative direction, however, was the same in both normal and low calcium conditions. These results indicated that the tallest and shortest hairs moved with equal angular deflection in the negative direction, while in the positive direction the shortest hair moved through a significantly greater angular deflection than the tallest hair. The implication of this finding is that the tip links contributed significantly to hair bundle motion in the positive direction only. |
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Authors:
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M D Eisen; R K Duncan; J C Saunders |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Hearing research Volume: 127 ISSN: 0378-5955 ISO Abbreviation: Hear. Res. Publication Date: 1999 Jan |
Date Detail:
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Created Date: 1999-04-05 Completed Date: 1999-04-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7900445 Medline TA: Hear Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 14-21 Citation Subset: IM |
Affiliation:
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Department of Otorhinolaryngology: Head and Neck Surgery, University of Pennsylvania, Philadelphia 19104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Cilia / physiology* Culture Media Hair Cells, Auditory / anatomy & histology, physiology* Movement |
| Grant Support | |
ID/Acronym/Agency:
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DC-00710/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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