Document Detail

The timSL mutant affects a restricted portion of the Drosophila melanogaster circadian cycle.
MedLine Citation:
PMID:  9783229     Owner:  NLM     Status:  MEDLINE    
The circadian rhythm genes period (per) and timeless (tim) are central to contemporary studies on Drosophila circadian rhythms. Mutations in these genes give rise to arrhythmic or period-altered phenotypes, and per and tim gene expression is under clock control. per and tim proteins (PER and TIM) also undergo circadian changes in level and phosphorylation state. The authors previously described a period-altering tim mutation, timSL, with allele-specific effects in different per backgrounds. This mutation also affected the TIM phosphorylation profile during the mid-late night. The authors show here that the single amino acid alteration in TIM-SL is indeed responsible for the phenotype, as a timSL transgene recapitulates the original mutant phenotype and shortens the period of perL flies by 3 h. The authors also show that this mutation has comparable effects in a light-dark cycle, as timSL also accelerates the activity offset during the mid-late night of perL flies. Importantly, timSL advances predominantly the mid-late night region of the perL phase response curve, consistent with the notion that this portion of the cycle is governed by unique rate-limiting steps. The authors propose that TIM and PER phosphorylation are normally rate determining during the mid-late night region of the circadian cycle.
J E Rutila; O Maltseva; M Rosbash
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biological rhythms     Volume:  13     ISSN:  0748-7304     ISO Abbreviation:  J. Biol. Rhythms     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-12-18     Completed Date:  1998-12-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8700115     Medline TA:  J Biol Rhythms     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  380-92     Citation Subset:  IM    
Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, MA 02454-9110, USA.
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MeSH Terms
Amino Acid Sequence
Behavior, Animal / physiology
Circadian Rhythm / physiology*
Drosophila Proteins*
Drosophila melanogaster / genetics*,  physiology*
Insect Proteins / genetics*
Mutation / physiology*
Nuclear Proteins / genetics
Period Circadian Proteins
Transgenes / genetics
Grant Support
Reg. No./Substance:
0/Drosophila Proteins; 0/Insect Proteins; 0/Nuclear Proteins; 0/PER protein, Drosophila; 0/Period Circadian Proteins; 0/timeless protein, Drosophila

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