| The thylakoid membrane protein ALB3 associates with the cpSecY-translocase in Arabidopsis thaliana. | |
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MedLine Citation:
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PMID: 12217076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The integration of light-harvesting chlorophyll proteins (LHCPs) into the thylakoid membrane requires the integral thylakoid membrane protein ALB3, a homologue of the bacterial cytoplasmic membrane protein YidC. In bacteria, YidC is associated with the SecY-translocase and facilitates the integration of Sec-dependent proteins into the plasma membrane. In addition, it is also involved in the insertion of Sec-independent proteins. In the present study we demonstrate, in Arabidopsis thaliana, that most ALB3 is a constituent of an oligomeric complex of approx. 180 kDa. In addition, we detected ALB3 in several higher-molecular-mass complexes (up to 700 kDa). Furthermore, we show that most ALB3 co-fractionates with cpSecY during gel-filtration analysis and blue native gel electrophoresis, suggesting an association of ALB3 with the cpSecY complex. A direct interaction of ALB3 with the cpSecY complex was demonstrated by co-immunoprecipitation experiments using digitonin-solubilized thylakoid membrane proteins and anti-cpSecY or anti-ALB3 antibodies. This result was further confirmed by electron microscopic co-immunolocalization of ALB3 and cpSecY. In addition, an association of ALB3 with the cpSecY complex was demonstrated directly by cross-linking experiments using the chemical cross-linker disuccinimidyl suberate. |
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Authors:
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Eva Klostermann; Imke Droste Gen Helling; Jean-Pierre Carde; Danja Schünemann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 368 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-05 Completed Date: 2003-02-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
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Languages: eng Pagination: 777-81 Citation Subset: IM |
Affiliation:
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Institut für Biologie III der RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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metabolism* Arabidopsis Proteins / chemistry*, metabolism* Chloroplasts / metabolism Chromatography, Gel Cross-Linking Reagents / pharmacology DNA, Complementary / metabolism Electrophoresis, Polyacrylamide Gel Immunohistochemistry Light-Harvesting Protein Complexes Membrane Proteins / metabolism* Microscopy, Electron Photosynthetic Reaction Center Complex Proteins / metabolism* Precipitin Tests Protein Binding Protein Transport Thylakoids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/ALBINO 3 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/Cross-Linking Reagents; 0/DNA, Complementary; 0/Light-Harvesting Protein Complexes; 0/Membrane Proteins; 0/Photosynthetic Reaction Center Complex Proteins; 0/SecY protein, Arabidopsis |
| Comments/Corrections | |
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