Document Detail


The thylakoid membrane protein ALB3 associates with the cpSecY-translocase in Arabidopsis thaliana.
MedLine Citation:
PMID:  12217076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The integration of light-harvesting chlorophyll proteins (LHCPs) into the thylakoid membrane requires the integral thylakoid membrane protein ALB3, a homologue of the bacterial cytoplasmic membrane protein YidC. In bacteria, YidC is associated with the SecY-translocase and facilitates the integration of Sec-dependent proteins into the plasma membrane. In addition, it is also involved in the insertion of Sec-independent proteins. In the present study we demonstrate, in Arabidopsis thaliana, that most ALB3 is a constituent of an oligomeric complex of approx. 180 kDa. In addition, we detected ALB3 in several higher-molecular-mass complexes (up to 700 kDa). Furthermore, we show that most ALB3 co-fractionates with cpSecY during gel-filtration analysis and blue native gel electrophoresis, suggesting an association of ALB3 with the cpSecY complex. A direct interaction of ALB3 with the cpSecY complex was demonstrated by co-immunoprecipitation experiments using digitonin-solubilized thylakoid membrane proteins and anti-cpSecY or anti-ALB3 antibodies. This result was further confirmed by electron microscopic co-immunolocalization of ALB3 and cpSecY. In addition, an association of ALB3 with the cpSecY complex was demonstrated directly by cross-linking experiments using the chemical cross-linker disuccinimidyl suberate.
Authors:
Eva Klostermann; Imke Droste Gen Helling; Jean-Pierre Carde; Danja Schünemann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  368     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-05     Completed Date:  2003-02-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  777-81     Citation Subset:  IM    
Affiliation:
Institut für Biologie III der RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry*,  metabolism*
Chloroplasts / metabolism
Chromatography, Gel
Cross-Linking Reagents / pharmacology
DNA, Complementary / metabolism
Electrophoresis, Polyacrylamide Gel
Immunohistochemistry
Light-Harvesting Protein Complexes
Membrane Proteins / metabolism*
Microscopy, Electron
Photosynthetic Reaction Center Complex Proteins / metabolism*
Precipitin Tests
Protein Binding
Protein Transport
Thylakoids / metabolism*
Chemical
Reg. No./Substance:
0/ALBINO 3 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/Cross-Linking Reagents; 0/DNA, Complementary; 0/Light-Harvesting Protein Complexes; 0/Membrane Proteins; 0/Photosynthetic Reaction Center Complex Proteins; 0/SecY protein, Arabidopsis
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